受自然启发的抗菌纳米拓扑结构和抗菌肽增强干细胞相容性，对抗植入相关感染,ACS Applied Nano Materials

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受自然启发的抗菌纳米拓扑结构和抗菌肽增强干细胞相容性，对抗植入相关感染
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-02-15 , DOI: 10.1021/acsanm.2c04913
Mohd Irill Ishak ₁ , Marcus Eales _{1,

2} , Laila Damiati ₃ , Xiayi Liu ₁ , Joshua Jenkins ₁ , Matthew J Dalby ₄ , Angela H Nobbs ₁ , Maxim G Ryadnov ₂ , Bo Su ₁

Affiliation

受自然启发的抗菌表面和抗菌肽（AMP）已成为对抗植入物相关感染的有前景的策略。在这项研究中，通过物理吸附将仿生抗菌肽功能化到纳米钉（NS）表面，目的是使其逐渐释放到局部环境中，从而增强对细菌生长的抑制。与纳米形貌相比，吸附在对照平面上的肽表现出不同的释放动力学，但两个表面都表现出优异的抗菌性能。用微摩尔浓度的肽进行官能化可抑制大肠杆菌在平坦表面上的生长、金黄色葡萄球菌在NS表面上的生长以及表皮葡萄球菌在平坦表面和NS表面上的生长。基于这些数据，我们提出了一种增强的抗菌机制，AMP可以使细菌细胞膜更容易受到纳米刺的影响，并且纳米刺引起的膜变形可以增加AMP膜插入的表面积。这些作用结合起来增强了杀菌活性。由于功能化纳米结构与干细胞具有高度生物相容性，因此它们成为下一代抗菌植入物表面的有希望的候选者。

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Enhanced and Stem-Cell-Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection

Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalized onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalization with peptide at micromolar concentrations inhibited Escherichia coli growth on the flat surface, Staphylococcus aureus growth on the NS surface, and Staphylococcus epidermidis growth on both the flat and NS surfaces. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalized nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.

更新日期：2023-02-15

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