UiO-66-Mediated Light-Driven Regeneration of Intracellular NADH in Clostridium tyrobutyricum to Strengthen Butyrate Production,ACS Sustainable Chemistry & Engineering

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UiO-66-Mediated Light-Driven Regeneration of Intracellular NADH in Clostridium tyrobutyricum to Strengthen Butyrate Production
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2023-02-14 , DOI: 10.1021/acssuschemeng.2c06628
Qianru Zhao ₁ , Yingfeng Li ₂ , Bowen Shen ₁ , Quanyu Zhao ₃ , Liying Zhu ₄ , Ling Jiang _{1,

5}

Affiliation

With the increasing depletion of petroleum and fossil resources, the development of green, low-carbon recycling systems has become the main goal for achieving carbon neutrality. Biohybrid photosynthesis systems have been confirmed to reduce carbon loss and promote microbial metabolism to achieve high yields of chemicals. Here, we used UiO-66, an excellent photocatalyst, to increase the intracellular pool of reduced nicotinamide adenine dinucleotide hydrogen (NADH) for butyrate production in Clostridium tyrobutyricum. The NADH/NAD⁺ ratio increased by 62% in the UiO-66 and C. tyrobutyricum hybrid system compared to the other groups under illumination, which resulted in a 25% increase in butyrate production (11.71 ± 0.16 vs 9.36 ± 0.14 g/L). The system improved the utilization rate of carbon sources and reduced carbon loss. Proteomic analysis also revealed that UiO-66 enhanced the intracellular NADH supply under illumination, leading to an increase in butyrate production. Thus, this system provides an outstanding strategy for improving the conversion of organic carbon into useful chemicals that helps to achieve energy savings and reduce emissions.

中文翻译：

UiO-66 介导的光驱动酪丁酸梭菌细胞内 NADH 再生以加强丁酸盐生产

随着石油和化石资源的日益枯竭，发展绿色、低碳的循环系统成为实现碳中和的主要目标。生物混合光合作用系统已被证实可以减少碳损失并促进微生物代谢以实现高产量的化学品。在这里，我们使用 UiO-66（一种出色的光催化剂）来增加细胞内还原型烟酰胺腺嘌呤二核苷酸氢 (NADH) 池，从而在酪丁酸梭菌中产生丁酸盐。UiO-66 和酪丁酸梭菌中的NADH/NAD ^{+比率增加了 62%}与光照下的其他组相比，混合系统导致丁酸盐产量增加 25%（11.71 ± 0.16 对 9.36 ± 0.14 g/L）。该系统提高了碳源的利用率，减少了碳损失。蛋白质组学分析还表明，UiO-66 在光照下增强了细胞内 NADH 的供应，从而导致丁酸盐产量增加。因此，该系统提供了一种出色的策略，可以提高有机碳向有用化学品的转化率，从而有助于实现节能和减少排放。

更新日期：2023-02-14

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