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High-Efficiency 3D DNA Walker Immobilized by a DNA Tetrahedral Nanostructure for Fast and Ultrasensitive Electrochemical Detection of MiRNA
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-14 , DOI: 10.1021/acs.analchem.2c04847 Xiao-Long Zhang 1 , Sha-Sha Li 1 , Yi-Jia Liu 1 , Wei-Wei Liu 1 , Ling-Qi Kong 1 , Ya-Qin Chai 1 , Xi-Liang Luo 2 , Ruo Yuan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-14 , DOI: 10.1021/acs.analchem.2c04847 Xiao-Long Zhang 1 , Sha-Sha Li 1 , Yi-Jia Liu 1 , Wei-Wei Liu 1 , Ling-Qi Kong 1 , Ya-Qin Chai 1 , Xi-Liang Luo 2 , Ruo Yuan 1
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Herein, by directly limiting the reaction space, an ingenious three-dimensional (3D) DNA walker (IDW) with high walking efficiency is developed for rapid and sensitive detection of miRNA. Compared with the traditional DNA walker, the IDW immobilized by the DNA tetrahedral nanostructure (DTN) brings stronger kinetic and thermodynamic favorability resulting from its improved local concentration and space confinement effect, accompanied by a quite faster reaction speed and much better walking efficiency. Once traces of target miRNA-21 react with the prelocked IDW, the IDW could be largely activated and walk on the interface of the electrode to trigger the cleavage of H2 with the assistance of Mg2+, resulting in the release of amounts of methylene blue (MB) labeled on H2 from the electrode surface and the obvious decrease of the electrode signal. Impressively, the IDW reveals a conversion efficiency as high as 9.33 × 108 in 30 min with a much fast reaction speed, which is at least five times beyond that of typical DNA walkers. Therefore, the IDW could address the inherent challenges of the traditional DNA walker easily: slow walking speed and low efficiency. Notably, the IDW as a DNA nanomachine was utilized to construct a sensitive sensing platform for rapid miRNA-21 detection with a limit of detection (LOD) of 19.8 aM and realize the highly sensitive assay of biomarker miRNA-21 in the total RNA lysates of cancer cell. The strategy thus helps in the design of a versatile nucleic acid conversion and signal amplification approach for practical applications in the areas of biosensing assay, DNA nanotechnology, and clinical diagnosis.
中文翻译：
由 DNA 四面体纳米结构固定的高效 3D DNA Walker，用于快速和超灵敏的 MiRNA 电化学检测
在此，通过直接限制反应空间，开发了一种具有高行走效率的巧妙三维 (3D) DNA 行走器 (IDW)，用于快速、灵敏地检测 miRNA。与传统的DNA步行器相比，由DNA四面体纳米结构（DTN）固定的IDW由于其改善的局部浓度和空间限制效应而带来更强的动力学和热力学有利性，伴随着更快的反应速度和更好的行走效率。一旦微量目标miRNA-21与预锁的IDW发生反应，IDW就可以被大量激活并在Mg 2+的帮助下在电极界面上行走以触发H2的裂解, 导致电极表面释放出大量标记在 H2 上的亚甲蓝 (MB), 电极信号明显下降。令人印象深刻的是，IDW 的转换效率高达 9.33 × 10 8在 30 分钟内以非常快的反应速度，这至少是典型 DNA 助行器的五倍。因此，IDW 可以轻松解决传统 DNA 行走器的固有挑战：行走速度慢、效率低。值得注意的是，IDW 作为 DNA 纳米机器被用于构建用于快速检测 miRNA-21 的灵敏传感平台，检测限 (LOD) 为 19.8 aM，实现了对总 RNA 裂解物中生物标志物 miRNA-21 的高灵敏度检测。癌细胞。因此，该策略有助于设计一种通用的核酸转换和信号放大方法，用于生物传感分析、DNA 纳米技术和临床诊断领域的实际应用。
更新日期：2023-02-14
中文翻译：
由 DNA 四面体纳米结构固定的高效 3D DNA Walker，用于快速和超灵敏的 MiRNA 电化学检测
在此，通过直接限制反应空间，开发了一种具有高行走效率的巧妙三维 (3D) DNA 行走器 (IDW)，用于快速、灵敏地检测 miRNA。与传统的DNA步行器相比，由DNA四面体纳米结构（DTN）固定的IDW由于其改善的局部浓度和空间限制效应而带来更强的动力学和热力学有利性，伴随着更快的反应速度和更好的行走效率。一旦微量目标miRNA-21与预锁的IDW发生反应，IDW就可以被大量激活并在Mg 2+的帮助下在电极界面上行走以触发H2的裂解, 导致电极表面释放出大量标记在 H2 上的亚甲蓝 (MB), 电极信号明显下降。令人印象深刻的是，IDW 的转换效率高达 9.33 × 10 8在 30 分钟内以非常快的反应速度，这至少是典型 DNA 助行器的五倍。因此，IDW 可以轻松解决传统 DNA 行走器的固有挑战：行走速度慢、效率低。值得注意的是，IDW 作为 DNA 纳米机器被用于构建用于快速检测 miRNA-21 的灵敏传感平台，检测限 (LOD) 为 19.8 aM，实现了对总 RNA 裂解物中生物标志物 miRNA-21 的高灵敏度检测。癌细胞。因此，该策略有助于设计一种通用的核酸转换和信号放大方法，用于生物传感分析、DNA 纳米技术和临床诊断领域的实际应用。
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