<br>用于水牛和牛牛奶生物合成和生产的硫解酶家族的比较基因组分析和乙酰辅酶 A 酰基转移酶 1 基因的功能表征,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于水牛和牛牛奶生物合成和生产的硫解酶家族的比较基因组分析和乙酰辅酶 A 酰基转移酶 1 基因的功能表征
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-02-13 , DOI: 10.1021/acs.jafc.2c07763
Tingxian Deng _{1,

2} , Jiyun Wu _{1,

3,

4} , Hamdy Abdel-Shafy ₅ , Xiaojie Wang ₁ , Haimiao Lv ₁ , Aftab Shaukat _{1,

5} , Xiang Zhou ₁ , Yang Zhou ₁ , Hui Sun _{1,

3,

4} , Pengfei Wei ₁ , Nan Sun ₁ , Qianzhi Huang ₁ , Linghua Xu ₁ , Miaoyu Liu ₁ , Yuxin Lin ₁ , Liguo Yang _{1,

6} , Guohua Hua _{1,

3,

4,

6}

Affiliation

牛和水牛分别是第一和第二大奶牛，提供了全球96%的奶制品。了解牛奶合成的机制对于开发提高牛奶产量的技术至关重要。硫解酶，也称为乙酰辅酶 A 乙酰转移酶 (ACAT)，是在脂质代谢中发挥重要作用的酶家族，包括 ACAT1、ACAT2、ACAA1、ACAA2 和 HADHB。我们目前的研究表明，这五个成员在包括水牛和牛在内的六种牲畜中是直系同源的。来自不同泌乳阶段的转录组数据分析表明， ACAA1在水牛和牛之间表现出不同的表达模式。免疫组织化学染色显示 ACAA1 主要位于这两种奶牛的乳腺上皮细胞中。 ACAA1的敲低通过调节牛和水牛的相关基因表达来抑制乳腺上皮细胞增殖以及甘油三酯和 β-酪蛋白的分泌。相反， ACAA1过度表达促进细胞增殖和甘油三酯分泌。最后，检测到三个新的 SNP（g.-681A>T、g.-23117C>T 和 g.-24348G>T），它们与地中海水牛的产奶性状显着相关。此外，位于启动子区的g.-681A>T突变显着改变了转录活性。我们的研究结果表明ACAA1在调节水牛和牛乳合成中发挥着关键作用，并为进一步了解奶牛泌乳生理学提供了基础信息。

"点击查看英文标题和摘要"

Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

Cattle and buffalo served as the first and second largest dairy animals, respectively, providing 96% milk products worldwide. Understanding the mechanisms underlying milk synthesis is critical to develop the technique to improve milk production. Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are an enzyme family that plays vital roles in lipid metabolism, including ACAT1, ACAT2, ACAA1, ACAA2, and HADHB. Our present study showed that these five members were orthologous in six livestock species including buffalo and cattle. Transcriptomic data analyses derived from different lactations stages showed that ACAA1 displayed different expression patterns between buffalo and cattle. Immunohistochemistry staining revealed that ACAA1 were dominantly located in the mammary epithelial cells of these two dairy animals. Knockdown of ACAA1 inhibited mammary epithelial cell proliferation and triglyceride and β-casein secretion by regulating related gene expressions in cattle and buffalo. In contrast, ACAA1 overexpression promoted cell proliferation and triglyceride secretion. Finally, three novel SNPs (g.-681A>T, g.-23117C>T, and g.-24348G>T) were detected and showed significant association with milk production traits of Mediterranean buffaloes. In addition, g.-681A>T mutation located in the promoter region changed transcriptional activity significantly. Our findings suggested that ACAA1 play a key role in regulating buffalo and cattle milk synthesis and provided basic information to further understand the dairy animal lactation physiology.

更新日期：2023-02-13

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南