年轻的外泌体生物纳米颗粒恢复老化受损的肌腱干/祖细胞功能和修复能力,Advanced Materials

当前位置： X-MOL 学术 › Adv. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

年轻的外泌体生物纳米颗粒恢复老化受损的肌腱干/祖细胞功能和修复能力
Advanced Materials ( IF 27.4 ) Pub Date : 2023-02-13 , DOI: 10.1002/adma.202211602
Shanshan Jin ₁ , Yu Wang ₁ , Xiaolan Wu ₁ , Zixin Li ₁ , Lisha Zhu ₁ , Yuting Niu ₂ , Yongsheng Zhou ₂ , Yan Liu ₁

Affiliation

衰老会损害肌腱干/祖细胞功能和肌腱稳态，然而，缺乏针对衰老引起的肌腱疾病的有效治疗方法。外泌体是天然衍生的含有生物活性分子的纳米颗粒，因此引起了组织工程和再生医学的极大兴趣。在这项研究中，表明人类乳牙脱落干细胞 (SHED-Exos) 分泌的年轻外泌体具有丰富的抗衰老信号。这些年轻的生物纳米颗粒可以减轻老化的肌腱干/祖细胞 (AT-SC) 的老化表型并保持其生肌能力。从机制上讲，SHED-Exos 调节组蛋白甲基化并抑制核因子-κB 以逆转 AT-SC 衰老。在自然衰老的小鼠模型中，SHED-Exo 生物纳米颗粒的全身给药可延缓肌腱退化。有趣的是，局部递送载有 SHED-Exos 的微球具有抗衰老表型，包括减少衰老细胞和减少异位骨形成，从而在功能和结构上挽救老年大鼠的内源性肌腱再生和修复能力。总的来说，SHED-Exos 作为天然生物活性纳米粒子，对衰老相关疾病具有广阔的转化和治疗潜力。

"点击查看英文标题和摘要"

Young Exosome Bio-Nanoparticles Restore Aging-Impaired Tendon Stem/Progenitor Cell Function and Reparative Capacity

Aging impairs tendon stem/progenitor cell function and tendon homeostasis, however, effective treatments for aging-induced tendon diseases are lacking. Exosomes are naturally derived nanoparticles that contain bioactive molecules, and therefore, have attracted great interest in tissue engineering and regenerative medicine. In this study, it is shown that young exosomes secreted by stem cells from human exfoliated deciduous teeth (SHED-Exos) possess abundant anti-aging signals. These young bio-nanoparticles can alleviate the aging phenotypes of aged tendon stem/progenitor cells (AT-SCs) and maintain their tenogenic capacity. Mechanistically, SHED-Exos modulate histone methylation and inhibit nuclear factor-κB to reverse AT-SC aging. In a naturally aging mouse model, systemic administration of SHED-Exo bio-nanoparticles retards tendon degeneration. Interestingly, local delivery of SHED-Exos-loaded microspheres confers anti-aging phenotypes, including reduced senescent cells and decreased ectopic bone formation, thereby functionally and structurally rescuing endogenous tendon regeneration and repair capacity in aged rats. Overall, SHED-Exos, as natural bioactive nanoparticles, have promising translational and therapeutic potential for aging-related diseases.

更新日期：2023-02-13

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南