Lignocellulosic biomass is the most abundant natural polymer on Earth, but the efficient fractionation and refinery of all its components remain challenging. Acidic deep eutectic solvents refining is a promising method, while it is likely to cause lignin condensation and carbohydrates degradation, especially at server operation conditions. Here we propose the use of acidic deep eutectic solvent (DES), choline chloride/p-toluenesulfonic acid assisted mechanochemical pretreatment (DM) for efficient lignocellulose fractionation at mild condition. Four representative lignocellulose, wheat straw, moso bamboo, poplar wood and pine wood were selected at varied milling time (3, 6 h) to assess the fractionation ability of this strategy. This DM pretreatment demonstrated a rather high cellulose retentions (∼90 %) and extent of delignification for wheat straw and bamboo biomass, which corresponds to a high extent of enzymatic hydrolysis (∼75.5 %) for sugar platform pursuing. The extracted lignin showed rather high content of β-O-4′ leakages due to the swelling effect of deep eutectic solvent and mild operation conditions. This work provided a promising strategy to fractionate lignocellulose using deep eutectic solvents with the goal of simultaneous cellulose hydrolysis and reactive lignin obtaining that is usually difficult to realize using traditional chemical fractionation approach.

木质纤维素生物质是地球上最丰富的天然聚合物，但其所有成分的高效分馏和精炼仍然具有挑战性。酸性低共熔溶剂精炼是一种很有前途的方法，但它可能会导致木质素凝结和碳水化合物降解，尤其是在服务器操作条件下。在这里，我们建议使用酸性低共熔溶剂 (DES)、氯化胆碱/对甲苯磺酸辅助机械化学预处理 (DM) 在温和条件下进行高效的木质纤维素分馏。在不同的研磨时间（3、6 小时）选择四种代表性的木质纤维素、麦秸、毛竹、杨木和松木来评估该策略的分馏能力。这种 DM 预处理表明小麦秸秆和竹子生物质具有相当高的纤维素保留率 (~90%) 和脱木质化程度，这对应于糖平台追求的高度酶水解 (~75.5%)。由于低共熔溶剂的溶胀作用和温和的操作条件，提取的木质素显示出相当高的 β-O-4' 泄漏含量。这项工作提供了一种使用低共熔溶剂分馏木质纤维素的有前途的策略，目标是同时进行纤维素水解和获得活性木质素，这通常很难使用传统的化学分馏方法实现。由于低共熔溶剂的溶胀作用和温和的操作条件，提取的木质素显示出相当高的 β-O-4' 泄漏含量。这项工作提供了一种使用低共熔溶剂分馏木质纤维素的有前途的策略，目标是同时进行纤维素水解和获得活性木质素，这通常很难使用传统的化学分馏方法实现。由于低共熔溶剂的溶胀作用和温和的操作条件，提取的木质素显示出相当高的 β-O-4' 泄漏含量。这项工作提供了一种使用低共熔溶剂分馏木质纤维素的有前途的策略，目标是同时进行纤维素水解和获得活性木质素，这通常很难使用传统的化学分馏方法实现。