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Fluorescence off–on nanosensor based on MoS2 nanosheets and oligonucleotides for the alternative detection of mercury(II) ions or silver(I) ions
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2023-02-10 , DOI: 10.1016/j.saa.2023.122479
Yonghui Xuan 1 , Xiang Li 2 , Changling Yan 3 , Gongke Wang 4
Affiliation  

As traditional methods for detection of heavy metal pollution in water involve complex procedures and require expensive equipment, there is a great deal of interest in the development of rapid and simple methods for determining heavy metal ions in water. Here, a nanobiosensor based on molybdenum disulphide (MoS2) nanosheets and fluorophore (FAM) labeled oligonucleotides was proposed, and fluorescence spectroscopy was adopted for detection of Hg2+ or Ag+ ions in aqueous solution. The principle underlying detection by the sensor involves the formation of T-Hg2+-T or C-Ag+-C mismatches by single-stranded DNA (ssDNA) rich in thymine (T) or cytosine (C), thereby forming stable double-stranded DNA (dsDNA) structures. By exploiting the different adsorption capacity of MoS2 nanosheets for ssDNA and dsDNA, when oligonucleotides were in a single chain state, MoS2 nanosheets possessed a strong adsorption capacity for ssDNA, resulting in fluorescence quenching of FAM. After the addition of Hg2+ or Ag+, ssDNA formed double chains structure, the fluorescence recovered due to the weak adsorption capacity of MoS2 nanosheets for dsDNA. Along this line, an “off-on” mode fluorescence nanobiosensor was designed to alternatively detect these two heavy metal ions in water. The sensor showed high sensitivity and excellent selectivity for both Hg2+ and Ag+ ions, with minimum detection limits of 6.8 nM and 8.9 nM, respectively.



中文翻译:

基于 MoS2 纳米片和寡核苷酸的荧光开关纳米传感器用于替代检测汞 (II) 离子或银 (I) 离子

由于检测水中重金属污染的传统方法程序复杂且需要昂贵的设备,因此开发快速简便的检测水中重金属离子的方法备受关注。在此,提出了一种基于二硫化钼 (MoS 2 ) 纳米片和荧光团 (FAM) 标记的寡核苷酸的纳米生物传感器,并采用荧光光谱法检测水溶液中的Hg 2+或 Ag +离子。传感器检测的基本原理涉及 T-Hg 2+ -T 或 C-Ag +的形成-C 通过富含胸腺嘧啶 (T) 或胞嘧啶 (C) 的单链 DNA (ssDNA) 错配,从而形成稳定的双链 DNA (dsDNA) 结构。利用MoS 2纳米片对ssDNA和dsDNA的不同吸附能力,当寡核苷酸处于单链状态时,MoS 2纳米片对ssDNA具有很强的吸附能力,导致FAM的荧光猝灭。加入Hg 2+或Ag +后,ssDNA形成双链结构,由于MoS 2吸附能力弱,荧光恢复用于 dsDNA 的纳米片。沿着这条线,设计了一种“关-开”模式的荧光纳米生物传感器来交替检测水中的这两种重金属离子。该传感器对 Hg 2+和 Ag +离子均表现出高灵敏度和出色的选择性,最低检测限分别为 6.8 nM 和 8.9 nM。

更新日期:2023-02-13
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