In the present study we report the development of an effective and relatively efficient protocol for protoplast-to-plant regeneration of parsnip (Pastinaca sativa L.) via indirect somatic embryogenesis. The regenerative potential of three open-pollinated and four hybrid cultivars was assessed. The protoplast isolation efficiency after digestion of source material in an enzyme mixture consisted of 1% cellulase Onozuka R-10 and 0.1% pectolyase Y-23 reached on average 3.6 × 10⁶ of cells per g of fresh mass. Protoplasts embedded in an alginate matrix and cultured in parsnip protoplast culture medium with phytosulfokine-α and putrescine reconstructed their cell wall and re-entered mitotic divisions. After the release from alginate, microcallus proliferated continuously on Gamborg B5 medium with vitamins supplemented with 100 nM of phytosulfokine-α. Indirect somatic embryogenesis occurred during the callus culture of cultivar ‘Półdługi biały’. The regenerated and acclimatized plants were morphologically similar to their donors and displayed no variation in the ploidy level.

在本研究中，我们报告了通过间接体细胞胚胎发生开发一种有效且相对高效的欧洲防风草 ( Pastinaca sativa L.) 原生质体到植物再生方案。评估了三个自由授粉和四个杂交品种的再生潜力。1%纤维素酶Onozuka R-10和0.1%果胶酶Y-23混合酶消化后原生质体分离效率平均达到3.6×10 ⁶每克新鲜质量的细胞数。原生质体包埋在藻酸盐基质中并在含植物磺基素-α 和腐胺的欧洲防风草原生质体培养基中培养，重建了它们的细胞壁并重新进入有丝分裂。从藻酸盐中释放后，微愈伤组织在 Gamborg B5 培养基上持续增殖，其中维生素补充了 100 nM 植物磺基素-α。间接体细胞胚胎发生发生在栽培品种“Półdługi biały”的愈伤组织培养过程中。再生和适应环境的植物在形态上与它们的供体相似，并且倍性水平没有变化。