Synthetic pesticides are a considerable issue in both terrestrial and aquatic habitats. Green pesticides derived from fungi, provide an efficient tools and alternative to chemicals. Secondary metabolites from Metarhizium anisopliae were tested against major disease mosquitos such as, Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus, as well as two non-target species, Eudrilus eugeniae and Artemia nauplii. After 24 h of treatment, M. anisopliae derived chemical constituent produced low LC₅₀ values, 54.678, 92.819, and 93.792 µg/ml on Aedes aegypti, for Anopheles stephensi, 80.933, 116.990 and 130.173 µg/ml, for Culex quinquefasciatus, 109.733, 196.540, 182.853 µg/ml in larvae, pupae, and adult, respectively. On the contrary, fungal metabolites are not hazardous to non-target species.. Midgut histology investigations revealed that M. anisopliae metabolites were less harmful to earthworms than chemical pesticides (Permethrin). The treatment of fungal crude extract exhibited minimal toxicity effects on gut tissues, normal epidermis, circular muscle, setae, mitochondrion, and intestinal lumen tissues. M. anisopliae crude metabolites showed a lower toxicity against the non-target species A. nauplii. Our results clearly shows minimal mortality rates were produced by the M. anisopliae derived chemical constituents, LC₅₀ and LC₉₀ values were, 455.947, 5098.233 μg/mL⁻1. No behavioral changes were observed during the treatment. The primary components of M.anisopliae crude extract identified by GC–MS studies were aucubin (14.356%) and 9, 12-Octadecadienoic acid (z,z)-(75.009%). The presence of key functional groups such as carboxylic acid, amides, and phenol groups was revealed by FT-IR spectral studies, which may be involved in mosquitocidal activity.

合成杀虫剂在陆地和水生栖息地都是一个相当大的问题。源自真菌的绿色农药，提供了一种有效的工具和化学品的替代品。Metarhizium anisopliae的次级代谢产物针对主要疾病蚊子进行了测试，例如史氏按蚊、埃及伊蚊和致密库蚊，以及两种非目标物种Eudrilus eugeniae和Artemia nauplii。处理 24 小时后，M. anisopliae衍生的化学成分在埃及伊蚊和斯氏按蚊上产生低 LC ₅₀值，分别为54.678、92.819 和 93.792 µg/ml, 80.933, 116.990 和 130.173 µg/ml, 对于Culex quinquefasciatus , 109.733, 196.540, 182.853 µg/ml in larvae, pupae, and adult, respectively. 相反，真菌代谢物对非目标物种没有危害。中肠组织学研究表明，绿僵菌代谢物对蚯蚓的危害小于化学杀虫剂（氯菊酯）。真菌粗提物的处理对肠道组织、正常表皮、环状肌肉、刚毛、线粒体和肠腔组织的毒性作用最小。M. anisopliae粗代谢物对非目标物种A. nauplii显示出较低的毒性。我们的结果清楚地表明M. anisopliae产生的死亡率最低衍生化学成分的 LC ₅₀和 LC ₉₀值分别为 455.947、5098.233 μg/mL ^- 1。在治疗期间未观察到行为变化。通过 GC-MS 研究鉴定的M.anisopliae粗提物的主要成分是桃叶珊瑚苷 (14.356%) 和 9, 12-十八碳二烯酸 (z,z)-(75.009%)。FT-IR 光谱研究揭示了羧酸、酰胺和酚基等关键官能团的存在，这些官能团可能与杀蚊活性有关。