Apple rubbery wood virus 1 (ARWV-1) and apple rubbery wood virus 2 (ARWV-2) are two negative-stranded RNA viruses first reported in apple trees and classified as two different species of the genus Rubodvirus. The geographic distribution of these viruses is mostly unknown. In 2021, during a field survey for viruses infecting pome fruit trees in Campania region (southern Italy) ARWV-1 and ARWV-2 were detected in single and double infections in pear and apple trees by RT-PCR and confirmed by sequencing of the amplicons. A degenerated primer pair, targeting a conserved domain in the RNA-dependent RNA polymerase of known rubodviruses, was designed to develop a RT-PCR assay able to diagnose these viruses at genus level. Preliminary results showed that the assay allowed the detection of ARWV-1 and ARWV-2 in single and mixed infection. Here, we report for the first time the natural occurrence of ARWV-1 in pear and the detection of ARWV-1 in apple and ARWV-2 in apple and pear in Italy, respectively.

苹果橡胶木病毒 1 (ARWV-1) 和苹果橡胶木病毒 2 (ARWV-2) 是在苹果树中首次报道的两种负链 RNA 病毒，被归类为Rubodvirus属的两个不同种. 这些病毒的地理分布大多是未知的。2021 年，在坎帕尼亚地区（意大利南部）对感染仁果树的病毒进行实地调查期间，通过 RT-PCR 在梨树和苹果树的单次和双重感染中检测到 ARWV-1 和 ARWV-2，并通过扩增子测序确认. 一对退化的引物以已知红病毒的 RNA 依赖性 RNA 聚合酶的保守结构域为目标，旨在开发一种能够在属水平上诊断这些病毒的 RT-PCR 测定法。初步结果表明，该检测方法可以检测单一和混合感染中的 ARWV-1 和 ARWV-2。在这里，我们首次报道了梨中 ARWV-1 的自然发生以及苹果中 ARWV-1 和意大利苹果和梨中 ARWV-2 的检测。