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Near-Infrared Ratiometric Hemicyanine-Based pH Fluorescence Probe with Bone Targetability for Monitoring Bone Resorption
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-06 , DOI: 10.1021/acs.analchem.2c04786 Yiling Meng 1, 2 , Delu Zhao 1 , Xingye Yang 3 , Yixuan Li 1 , Bohui Liu 1 , Zijie Zhang 1 , Shuyue Cui 1 , Fulan Wei 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-06 , DOI: 10.1021/acs.analchem.2c04786 Yiling Meng 1, 2 , Delu Zhao 1 , Xingye Yang 3 , Yixuan Li 1 , Bohui Liu 1 , Zijie Zhang 1 , Shuyue Cui 1 , Fulan Wei 1
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Accurate detection of bone resorption is extremely important in the orthodontic treatment process as it can provide a basis for clinical treatment strategies. Recently, pH-responsive fluorescence probes have received tremendous attention in bone resorption monitoring owing to their high sensitivity, good specificity, and in situ and real-time detection capabilities, but there are still some shortcomings like the increase in the risk of osteonecrosis of the jaw by use of bisphosphonate as the bone-targeting moiety and the insufficient monitoring accuracy due to susceptibility to interference. Herein, we designed and synthesized a near-infrared ratiometric hemicyanine-based pH fluorescence probe (Hcy-Asp6) with fluorescence-imaging and pH-determining capabilities, and bone targetability for more reliably and safely monitoring the bone resorption in orthodontic treatment. In vitro optical performance tests of Hcy-Asp6 revealed that the probe had high sensitivity, excellent photostability, reversibility, and strong resistance to interference, and the probe suggested excellent bone-binding ability and biocompatibility in the bone-targeting evaluation and the cytotoxicity test. Furthermore, in vitro and in vivo bone resorption monitoring assays demonstrated that this probe can detect bone resorption by fluorescence imaging and quantitative monitoring of pH associated with the bone resorption. Thus, the results indicated that this probe possessing bone targetability and accurate bone resorption-monitoring capability has an extraordinarily great clinical potential to be employed for real-time monitoring of bone resorption in orthodontic treatment and could also serve as a reference in bone resorption monitoring for other bone resorption-related diseases.
中文翻译：
具有骨靶向性的近红外比例半花青基 pH 荧光探针用于监测骨吸收
骨吸收的准确检测在正畸治疗过程中极为重要，因为它可以为临床治疗策略提供依据。近年来，pH响应荧光探针以其灵敏度高、特异性好、原位实时检测等优点在骨吸收监测中受到极大关注，但仍存在增加骨坏死风险等缺点。通过使用双膦酸盐作为骨靶向部分和由于易受干扰而导致的监测准确性不足。在此，我们设计并合成了一种具有荧光成像和 pH 测定能力的近红外比例半花青素 pH 荧光探针 (Hcy-Asp6)，和骨靶向性，以便更可靠和安全地监测正畸治疗中的骨吸收。Hcy-Asp6的体外光学性能测试表明，该探针具有高灵敏度、优异的光稳定性、可逆性、抗干扰性强等特点，在骨靶向评价和细胞毒性试验中具有良好的骨结合能力和生物相容性。此外，体外和体内骨吸收监测试验表明，该探针可以通过荧光成像和与骨吸收相关的 pH 值的定量监测来检测骨吸收。因此，
更新日期：2023-02-06
中文翻译：
具有骨靶向性的近红外比例半花青基 pH 荧光探针用于监测骨吸收
骨吸收的准确检测在正畸治疗过程中极为重要，因为它可以为临床治疗策略提供依据。近年来，pH响应荧光探针以其灵敏度高、特异性好、原位实时检测等优点在骨吸收监测中受到极大关注，但仍存在增加骨坏死风险等缺点。通过使用双膦酸盐作为骨靶向部分和由于易受干扰而导致的监测准确性不足。在此，我们设计并合成了一种具有荧光成像和 pH 测定能力的近红外比例半花青素 pH 荧光探针 (Hcy-Asp6)，和骨靶向性，以便更可靠和安全地监测正畸治疗中的骨吸收。Hcy-Asp6的体外光学性能测试表明，该探针具有高灵敏度、优异的光稳定性、可逆性、抗干扰性强等特点，在骨靶向评价和细胞毒性试验中具有良好的骨结合能力和生物相容性。此外，体外和体内骨吸收监测试验表明，该探针可以通过荧光成像和与骨吸收相关的 pH 值的定量监测来检测骨吸收。因此，
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