Miniature Hierarchical DNA Hybridization Circuit for Amplified Multiplexed MicroRNA Imaging,Analytical Chemistry

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Miniature Hierarchical DNA Hybridization Circuit for Amplified Multiplexed MicroRNA Imaging
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-06 , DOI: 10.1021/acs.analchem.2c05373
Ruomeng Li ₁ , Fengzhe Li ₁ , Yanping Zhang ₁ , Yuqiu He ₁ , Yushi Wang ₁ , Fuan Wang _{1,

2}

Affiliation

Accurate diagnosis requires the development of multiple-guaranteed DNA circuits. Nevertheless, for reliable multiplexed molecular imaging, existing DNA circuits are limited by poor cell-delivering homogeneity due to their cumbersome and dispersive reactants. Herein, we developed a compact-yet-efficient hierarchical DNA hybridization (HDH) circuit for in situ simultaneous analysis of multiple miRNAs, which could be further exploited for specifically discriminating cancer cells from normal ones. By integrating the traditional hybridization chain reaction and catalytic hairpin assembly reactants into two highly organized hairpins, the HDH circuit is fitted with condensed components and multiple response domains, thus permitting the programmable multiple microRNA-guaranteed sequential activations and the localized cascaded signal amplification. The synergistic multi-recognition and amplification features of the HDH circuit facilitate the magnified detection of multiplex endogenous miRNAs in living cells. The in vitro and cellular imaging experimental results revealed that the HDH circuit displayed a reliable sensing performance with high selective cell-identification capacity. We anticipate that this compact design can provide a powerful toolkit for accurate diagnostics and pathological evolution.

中文翻译：

用于放大多重 MicroRNA 成像的微型分级 DNA 杂交电路

准确的诊断需要开发多重保证的 DNA 电路。然而，对于可靠的多重分子成像，现有的 DNA 电路由于其笨重和分散的反应物而受到细胞输送均匀性差的限制。在此，我们开发了一种紧凑而高效的分层 DNA 杂交 (HDH) 电路，用于原位同时分析多个 miRNA，可进一步用于特异性区分癌细胞和正常细胞。通过将传统的杂交链式反应和催化发夹组装反应物集成到两个高度组织化的发夹中，HDH 电路配备了浓缩组件和多个响应域，从而允许可编程的多个 microRNA 保证顺序激活和局部级联信号放大。HDH 电路的协同多重识别和放大特性有助于放大检测活细胞中的多重内源性 miRNA。体外和细胞成像实验结果表明，HDH 电路显示出可靠的传感性能和高选择性细胞识别能力。我们预计这种紧凑的设计可以为准确诊断和病理进化提供强大的工具包。

更新日期：2023-02-06

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