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Exogenous interference and autofluorescence-free ratiometric aptasensor for detection of OTA based on dual-colored persistent luminescence nanoparticles
Food Chemistry ( IF 8.5 ) Pub Date : 2023-02-01 , DOI: 10.1016/j.foodchem.2023.135611
Jing-Xuan Guo 1 , Lu-Ming Pan 2 , Meng-Chao Wang 3 , Li-Jian Chen 3 , Xu Zhao 3
Food Chemistry ( IF 8.5 ) Pub Date : 2023-02-01 , DOI: 10.1016/j.foodchem.2023.135611
Jing-Xuan Guo 1 , Lu-Ming Pan 2 , Meng-Chao Wang 3 , Li-Jian Chen 3 , Xu Zhao 3
Affiliation
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Accurate and sensitive detection of ochratoxin A (OTA) is highly necessary due to its high carcinogenicity, teratogenicity and mutagenicity. Herein, we reported an exogenous interference and autofluorescence-free ratiometric aptasensor based on dual-colored persistent luminescent nanoparticles for precise detection of OTA. Green-emitting ZnGeO:Mn bonded with OTA aptamer and BHQ1-modified complementary base was acted as detection and specific recognition probe (ZGM@BHQ1). Quaternary ammonium modified ZnGaGeO:Cr with red emission was employed as reference probe and further bonded to ZGM@BHQ1 through electrostatic interaction to construct the ratiometric aptasensor. The developed ratiometric aptasensor was free from real-time excitation, external interference and autofluorescence and gave low detection limit of 3.4 pg mL−1 , wide linearity in the range of 0.01–50 ng mL−1 and high precision of 3.1 % (11 replicate determinations, at 1 ng mL−1 level). The applicability of the aptasensor was successfully demonstrated by analyzing OTA in in grain samples with recoveries of 97.6 %-105.2 %.
中文翻译:
外源干涉和无自荧光比率适配传感器,用于基于双色持续发光纳米颗粒的 OTA 检测
准确、灵敏地检测赭曲霉毒素 A (OTA) 具有很高的致癌性、致畸性和致突变性,因此非常必要。在此,我们报道了一种基于双色持续发光纳米颗粒的外源干扰和无自发荧光比率适配体传感器,用于精确检测 OTA。与 OTA 适配体和 BHQ1 修饰的互补碱基键合的绿色发光 ZnGeO:Mn 作为检测和特异性识别探针 (ZGM@BHQ1)。采用具有红色发射的季铵修饰的 ZnGaGeO:Cr 作为参考探针,并通过静电相互作用进一步键合到ZGM@BHQ1以构建比率式适配体传感器。开发的比率式适配体传感器不受实时激发、外部干扰和自发荧光的影响,检测限低至 3.4 pg mL-1,线性范围为 0.01–50 ng mL-1,精度高达 3.1%(11 次重复测定,1 ng mL-1 水平)。通过分析谷物样品中的 OTA 成功证明了 aptasensor 的适用性,回收率为 97.6 %-105.2 %。
更新日期:2023-02-01
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
外源干涉和无自荧光比率适配传感器,用于基于双色持续发光纳米颗粒的 OTA 检测
准确、灵敏地检测赭曲霉毒素 A (OTA) 具有很高的致癌性、致畸性和致突变性,因此非常必要。在此,我们报道了一种基于双色持续发光纳米颗粒的外源干扰和无自发荧光比率适配体传感器,用于精确检测 OTA。与 OTA 适配体和 BHQ1 修饰的互补碱基键合的绿色发光 ZnGeO:Mn 作为检测和特异性识别探针 (ZGM@BHQ1)。采用具有红色发射的季铵修饰的 ZnGaGeO:Cr 作为参考探针,并通过静电相互作用进一步键合到ZGM@BHQ1以构建比率式适配体传感器。开发的比率式适配体传感器不受实时激发、外部干扰和自发荧光的影响,检测限低至 3.4 pg mL-1,线性范围为 0.01–50 ng mL-1,精度高达 3.1%(11 次重复测定,1 ng mL-1 水平)。通过分析谷物样品中的 OTA 成功证明了 aptasensor 的适用性,回收率为 97.6 %-105.2 %。