细胞冷冻保存的液滴生成、玻璃化和加热：综述,ACS Biomaterials Science & Engineering

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细胞冷冻保存的液滴生成、玻璃化和加热：综述
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-02-06 , DOI: 10.1021/acsbiomaterials.2c01087
Mengdong Cui _{1,

2,

3} , Taijie Zhan _{1,

2,

3} , Jiamin Yang _{1,

2,

3} , Hangyu Dang _{1,

2,

3} , Guoliang Yang _{1,

2,

3} , Hengxin Han _{1,

2,

3} , Linfeng Liu _{1,

2,

3} , Yi Xu _{1,

2,

3}

Affiliation

冷冻保存是目前转化医学的关键步骤，可为生殖医学、再生医学和细胞治疗的临床应用提供新思路。液滴玻璃化打印技术具有冷冻保护剂浓度低、降温速度快、操作简便等优点，在冷冻保存领域受到广泛关注。本综述总结了液滴的产生、玻璃化和加热方法。喷墨打印、微阀打印、声学打印等液滴生成技术已应用于低温保存领域。液滴玻璃化包括液氮直接接触玻璃化和液滴固体表面玻璃化。液滴玻璃化的局限性（液氮污染、液滴蒸发、气膜抑制传热、结霜）及解决方法进行了讨论。此外，外部物理场升温法与传统水浴法的比较表明，在微滴的自动快速升温方面可以实现更好的应用。液滴玻璃化技术与外部物理场加温技术相结合，有望实现高通量和自动化冷冻保存，在生物医学和再生医学领域具有广阔的前景。

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Droplet Generation, Vitrification, and Warming for Cell Cryopreservation: A Review

Cryopreservation is currently a key step in translational medicine that could provide new ideas for clinical applications in reproductive medicine, regenerative medicine, and cell therapy. With the advantages of a low concentration of cryoprotectant, fast cooling rate, and easy operation, droplet-based printing for vitrification has received wide attention in the field of cryopreservation. This review summarizes the droplet generation, vitrification, and warming method. Droplet generation techniques such as inkjet printing, microvalve printing, and acoustic printing have been applied in the field of cryopreservation. Droplet vitrification includes direct contact with liquid nitrogen vitrification and droplet solid surface vitrification. The limitations of droplet vitrification (liquid nitrogen contamination, droplet evaporation, gas film inhibition of heat transfer, frosting) and solutions are discussed. Furthermore, a comparison of the external physical field warming method with the conventional water bath method revealed that better applications can be achieved in automated rapid warming of microdroplets. The combination of droplet vitrification technology and external physical field warming technology is expected to enable high-throughput and automated cryopreservation, which has a promising future in biomedicine and regenerative medicine.

更新日期：2023-02-06

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