Poly(N-acryloyl glycinamide-co-N-acryloxysuccinimide) Nanoparticles: Tunable Thermo-Responsiveness and Improved Bio-Interfacial Adhesion for Cell Function Regulation,ACS Applied Materials & Interfaces

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Poly(N-acryloyl glycinamide-co-N-acryloxysuccinimide) Nanoparticles: Tunable Thermo-Responsiveness and Improved Bio-Interfacial Adhesion for Cell Function Regulation
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-02-05 , DOI: 10.1021/acsami.2c22267
Yueyi Tian ₁ , Jiahui Lai ₁ , Chen Li ₁ , Jialin Sun ₁ , Kang Liu ₂ , Chuanzhuang Zhao ₂ , Mingming Zhang ₁

Affiliation

Poly(N-acryloyl glycinamide) (PNAGA) can form high-strength hydrogen bonds (H-bonds) through the dual amide motifs in the side chain, allowing the polymer to exhibit gelation behavior and an upper critical solution temperature (UCST) property. These features make PNAGA a candidate platform for biomedical devices. However, most applications focused on PNAGA hydrogels, while few focused on PNAGA nanoparticles. Improving the UCST tunability and bio-interfacial adhesion of the PNAGA nanoparticles may expand their applications in biomedical fields. To address the issues, we established a reactive H-bond-type P(NAGA-co-NAS) copolymer via reversible addition–fragmentation chain transfer polymerization of NAGA and N-acryloxysuccinimide (NAS) monomers. The UCST behaviors and the bio-interfacial adhesion toward the proteins and cells along with the potential application of the copolymer nanoparticles were investigated in detail. Taking advantage of the enhanced H-bonding and reactivity, the copolymer exhibited a tunable UCST in a broad temperature range, showing thermo-reversible transition between nanoparticles (PNPs) and soluble chains; the PNPs efficiently bonded proteins into nano-biohybrids while keeping the secondary structure of the protein, and more importantly, they also exhibited good adhesion ability to the cell membrane and significantly inhibited cell-specific propagation. These features suggest broad prospects for the P(NAGA-co-NAS) nanoparticles in the fields of biosensors, protein delivery, cell surface decoration, and cell-specific function regulation.

中文翻译：

聚（N-丙烯酰基甘氨酰胺-co-N-丙烯酰氧基琥珀酰亚胺）纳米颗粒：可调节的热响应性和改进的生物界面粘附性用于细胞功能调节

聚（N-丙烯酰基甘氨酰胺）（PNAGA）可以通过侧链中的双酰胺基序形成高强度氢键（H 键），从而使聚合物表现出凝胶行为和上限临界溶解温度（UCST）特性。这些特性使 PNAGA 成为生物医学设备的候选平台。然而，大多数应用都集中在 PNAGA 水凝胶上，而很少有人关注 PNAGA 纳米粒子。提高 PNAGA 纳米粒子的 UCST 可调性和生物界面粘附性可能会扩大其在生物医学领域的应用。为了解决这些问题，我们通过NAGA 和N的可逆加成-断裂链转移聚合，建立了反应性 H 键型 P(NAGA- co -NAS) 共聚物-丙烯酰氧基琥珀酰亚胺 (NAS) 单体。详细研究了 UCST 行为和对蛋白质和细胞的生物界面粘附以及共聚物纳米粒子的潜在应用。利用增强的氢键和反应性，该共聚物在较宽的温度范围内表现出可调的 UCST，显示出纳米颗粒 (PNP) 和可溶性链之间的热可逆转变；PNPs有效地将蛋白质键合成纳米生物杂交体，同时保持蛋白质的二级结构，更重要的是，它们还表现出良好的细胞膜粘附能力，显着抑制细胞特异性增殖。这些特征表明 P(NAGA- co-NAS)纳米粒子在生物传感器、蛋白质传递、细胞表面装饰、细胞特异性功能调控等领域的应用。

更新日期：2023-02-05

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