双适配子辅助比例 SERS 生物传感器用于超灵敏和精确识别乳腺癌外泌体,ACS Sensors

当前位置： X-MOL 学术 › ACS Sens. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

双适配子辅助比例 SERS 生物传感器用于超灵敏和精确识别乳腺癌外泌体
ACS Sensors ( IF 8.2 ) Pub Date : 2023-02-01 , DOI: 10.1021/acssensors.2c02587
Qi Zhang ₁ , Ruofei Ma ₁ , Yingzhi Zhang ₁ , Jing Zhao ₁ , Yue Wang ₁ , Zhangrun Xu ₁

Affiliation

由于乳腺癌的异质性，其早期准确诊断仍然是一个挑战。外泌体携带丰富的遗传物质和蛋白质，是早期癌症检测的理想生物标志物。在此，使用规则排列的 Au@Ag 纳米粒子/氧化石墨烯 (Au@Ag NPs/GO) 底物和 4-硝基苯硫酚 (4-NTP) 分子作为外泌体检测的比例表面增强拉曼散射 (SERS) 生物传感器构建内部标准。两种过表达蛋白（上皮细胞粘附分子和人表皮生长因子受体 2）的适体通过短的互补 DNA 连接，在 3' 末端修饰罗丹明 X，形成 V 形双链 DNA，附着在表面Au@Ag NPs/GO 底物用于选择性识别乳腺癌细胞来源的外泌体。在外泌体存在的情况下，发生竞争反应，形成V型双链DNA/外泌体复合物，V型双链DNA与SERS底物分离。罗丹明X在V型双链DNA上的SERS信号随着外泌体浓度的增加而降低，而4-NTP在底物上的SERS信号保持稳定。比例 SERS 策略在 GO 层上提供了巨大的电磁增强和丰富的 DNA 吸附位点，实现了 2.7 × 10 的宽检测范围罗丹明X在V型双链DNA上的SERS信号随着外泌体浓度的增加而降低，而4-NTP在底物上的SERS信号保持稳定。比例 SERS 策略在 GO 层上提供了巨大的电磁增强和丰富的 DNA 吸附位点，实现了 2.7 × 10 的宽检测范围罗丹明X在V型双链DNA上的SERS信号随着外泌体浓度的增加而降低，而4-NTP在底物上的SERS信号保持稳定。比例 SERS 策略在 GO 层上提供了巨大的电磁增强和丰富的 DNA 吸附位点，实现了 2.7 × 10 的宽检测范围²至 2.7 × 10 ^{8 个}颗粒/mL，超低检测限低至 1.5 × 10 ^{2 个}颗粒/mL，无需任何核酸扩增。特别是，所提出的方法在早期癌症诊断中具有重要应用，因为它可以准确识别临床血清样本中的乳腺癌细胞来源的外泌体，并可以区分胰腺癌患者和健康个体。

"点击查看英文标题和摘要"

Dual-Aptamer-Assisted Ratiometric SERS Biosensor for Ultrasensitive and Precise Identification of Breast Cancer Exosomes

Due to the heterogeneity of breast cancer, its early accurate diagnosis remains a challenge. Exosomes carry abundant genetic materials and proteins and are ideal biomarkers for early cancer detection. Herein, a ratiometric surface-enhanced Raman scattering (SERS) biosensor for exosome detection was constructed using a regularly arranged Au@Ag nanoparticles/graphene oxide (Au@Ag NPs/GO) substrate with 4-nitrothiophenol (4-NTP) molecules as an internal standard. Aptamers of two overexpressed proteins (epithelial cell adhesion molecule and human epidermal growth factor receptor 2) were linked by a short complementary DNA with rhodamine X modified at the 3′-terminal to form V-shaped double-stranded DNA, which attached to the surface of Au@Ag NPs/GO substrate for the selective recognition of breast cancer cell-derived exosomes. In the presence of exosomes, a competitive reaction occurred, resulting in the formation of the V-shaped double-stranded DNA/exosomes complex, and the V-shaped double-stranded DNA separated from the SERS substrate. The SERS signal of rhodamine X on the V-shaped double-stranded DNA decreased with the concentration of exosomes increasing, whereas the SERS signal of 4-NTP on the substrate remained stable. The ratiometric SERS strategy provides huge electromagnetic enhancement and abundant DNA adsorbing sites on the GO layer, achieving a wide detection range of 2.7 × 10² to 2.7 × 10⁸ particles/mL and an ultralow limit of detection down to 1.5 × 10² particles/mL, without the requirement of any nucleic acid amplification. Particularly, the proposed method has significant applications in early cancer diagnosis as it can accurately identify breast cancer cell-derived exosomes in clinical serum samples and can differentiate pancreatic cancer patients and healthy individuals.

更新日期：2023-02-01

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南