Electrochemiluminescence (ECL) has been widely applied in imaging owing to features that distinguish it from other microscopic techniques and electrochemical methods, including its high signal-to-noise ratio, remarkable sensitivity, wide linear range, high spatiotemporal resolution, and near-zero background light. Imaging technology based on ECL has been used in the fields of immunosensing, pathological cell detection, and drug analysis. Additionally, its simple operation and ability to detect dynamic processes and catalytic sites strengthen its potential for research on material surfaces and interfaces, in vivo biological analysis, and cell visualization. At the same time, the emergence of a variety of nanomaterials and new microscopic analysis equipment has further promoted the development of high-resolution ECL imaging technology. This paper introduces the development of ECL technology and the mechanisms of the main ECL systems. It then describes various forms of ECL imaging methods, and reviews research progress on ECL imaging technology in the fields of single-particle imaging, fingerprint structure analysis, and single-cell microscopic imaging. Finally, the authors offer their views about the prospects for ECL imaging technology.

中文翻译：

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电化学发光成像分析的最新进展


电化学发光 （ECL） 因其区别于其他显微技术和电化学方法的特点，包括高信噪比、卓越的灵敏度、宽线性范围、高时空分辨率和接近零的背景光，因此在成像中得到了广泛的应用。基于 ECL 的成像技术已用于免疫传感、病理细胞检测和药物分析领域。此外，其简单的操作和检测动态过程和催化位点的能力增强了其在材料表面和界面、体内生物分析和细胞可视化研究方面的潜力。同时，各种纳米材料和新型显微分析设备的出现进一步推动了高分辨率 ECL 成像技术的发展。本文介绍了 ECL 技术的发展和主要 ECL 系统的机制。然后介绍了各种形式的 ECL 成像方法，并综述了 ECL 成像技术在单颗粒成像、指纹结构分析和单细胞显微成像领域的研究进展。最后，作者对 ECL 成像技术的前景提出了他们的看法。