2-Bromopropane (2-BP) is a colorless liquid at room temperature and is used in closed systems in factories, mainly as an intermediate for medicines, pesticides, and other chemicals. However, the carcinogenicity of 2-BP is still unknown. The CByB6F1-Tg(HRAS)2Jic (rasH2) transgenic mouse model has been established as an alternative to long-term studies (1.5 years—lifetime) to detect carcinogenicity in as short a time as six months. We performed a 26-week inhalation exposure study of 2-BP using the rasH2 mouse model. Male and female rasH2 mice were exposed to 0, 67, 200, or 600 ppm of 2-BP for 6 h/day, 5 days/week for 26 weeks. All tissues and blood were collected and subjected to biological and histopathological analyses. The results showed a concentration-dependent increase in lung tumor development in male and female rasH2 mice exposed by inhalation to 2-BP, which was significant by Peto’s and Poly-3 trend tests. Furthermore, in male rasH2 mice, 2-BP was found to be a testicular toxin. This study is the first to demonstrate that 2-BP is carcinogenic in male and female mice and a testicular toxin in male mice using the rasH2 mouse model.

2-溴丙烷（2-BP）在室温下是无色液体，用于工厂的封闭系统中，主要作为医药、农药和其他化学品的中间体。然而，2-BP的致癌性仍不清楚。 CByB6F1-Tg(HRAS)2Jic (rasH2) 转基因小鼠模型已被建立作为长期研究（1.5 年——一生）的替代方案，可在短短六个月内检测致癌性。我们使用 rasH2 小鼠模型进行了为期 26 周的 2-BP 吸入暴露研究。雄性和雌性 rasH2 小鼠暴露于 0、67、200 或 600 ppm 的 2-BP，每天 6 小时，每周 5 天，持续 26 周。收集所有组织和血液并进行生物学和组织病理学分析。结果显示，吸入 2-BP 的雄性和雌性 rasH2 小鼠的肺部肿瘤发展呈浓度依赖性增加，Peto 和 Poly-3 趋势测试显示这一点很显着。此外，在雄性 rasH2 小鼠中，发现 2-BP 是一种睾丸毒素。这项研究首次使用 rasH2 小鼠模型证明 2-BP 在雄性和雌性小鼠中具有致癌性，在雄性小鼠中具有睾丸毒素。