Dry Chemistry-Based Bipolar Electrochemiluminescence Immunoassay Device for Point-of-Care Testing of Alzheimer-Associated Neuronal Thread Protein,Analytical Chemistry

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Dry Chemistry-Based Bipolar Electrochemiluminescence Immunoassay Device for Point-of-Care Testing of Alzheimer-Associated Neuronal Thread Protein
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-01-31 , DOI: 10.1021/acs.analchem.2c05164
Yi Liang ₁ , Kaifa Xue ₁ , Yanyang Shi ₁ , Tingting Zhan ₁ , Wei Lai ₁ , Chunsun Zhang ₁

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In this study, we developed, for the first time, a novel dry chemistry-based bipolar electrochemiluminescence (ECL) immunoassay device for point-of-care testing (POCT) of Alzheimer-associated neuronal thread protein (AD7c-NTP), where the ECL signals were automatically collected and analyzed after the sample and buffer solutions were manually added onto the immunosensor. The proposed immunoassay device contains an automatic ECL analyzer and a dry chemistry-based ECL immunosensor fabricated with a screen-printed fiber material-based chip and a three-dimensional (3D)-printed shell. Each pad of the fiber material-based chip was premodified with certain reagents for immunoreaction and then assembled to form the ECL immunosensor. The self-enhanced ECL of the Ru(II)-poly-l-lysine complex and the lateral flow fiber material-based chip make the addition of coreactants and repeated flushing unnecessary. Only the sample and buffer solutions are added to the ECL immunosensor, and the process of ECL detection can be completed in about 6 min using the proposed automatic ECL analyzer. Under optimized conditions, the linear detection range for AD7c-NTP was 1 to 10⁴ pg/mL, and the detection limit was 0.15 pg/mL. The proposed ECL immunoassay device had acceptable selectivity, stability, and reproducibility and had been successfully applied to detect AD7c-NTP levels in human urine. In addition, the accurate detection of AD7c-NTP and duplex detection of AD7c-NTP and apolipoprotein E ε4 gene were also validated. It is believed that the proposed ECL immunoassay device may be a candidate for future POCT applications.

中文翻译：

基于干化学的双极电化学发光免疫测定装置用于阿尔茨海默病相关神经元线蛋白的即时检测

在这项研究中，我们首次开发了一种新型的基于干化学的双极电化学发光 (ECL) 免疫测定装置，用于阿尔茨海默相关神经元丝蛋白 (AD7c-NTP) 的即时检测 (POCT)，其中在将样品和缓冲溶液手动添加到免疫传感器上后，自动收集和分析 ECL 信号。拟议的免疫测定装置包含一个自动 ECL 分析仪和一个基于干化学的 ECL 免疫传感器，该传感器由基于丝网印刷纤维材料的芯片和三维 (3D) 打印外壳制成。基于纤维材料的芯片的每个垫都用特定的免疫反应试剂进行预修饰，然后组装形成 ECL 免疫传感器。Ru(II)-poly- l的自增强 ECL-赖氨酸复合物和基于侧流纤维材料的芯片使得无需添加共反应物和重复冲洗。仅将样品和缓冲溶液添加到 ECL 免疫传感器中，使用建议的自动 ECL 分析仪可以在约 6 分钟内完成 ECL 检测过程。在优化条件下，AD7c-NTP 的线性检测范围为 1 至 10 ⁴pg/mL，检出限为 0.15 pg/mL。所提出的 ECL 免疫测定装置具有可接受的选择性、稳定性和重现性，并已成功应用于检测人尿液中的 AD7c-NTP 水平。此外，还验证了AD7c-NTP的准确检测和AD7c-NTP与载脂蛋白Eε4基因的双重检测。据信，所提出的 ECL 免疫测定装置可能是未来 POCT 应用的候选者。

更新日期：2023-01-31

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