纳米线尖端的避雷针效应增强电穿孔和电化学氧化：消除细胞内抗生素抗性基因的有效策略,ACS Nano

当前位置： X-MOL 学术 › ACS Nano › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

纳米线尖端的避雷针效应增强电穿孔和电化学氧化：消除细胞内抗生素抗性基因的有效策略
ACS Nano ( IF 15.8 ) Pub Date : 2023-01-30 , DOI: 10.1021/acsnano.2c11811
Hai Liu ₁ , Wei Huang ₁ , Yang Yu ₁ , Da Chen ₁

Affiliation

由于抗生素抗性细菌 (ARB) 细胞成分的竞争性氧化，传统的氧化消毒方法通常无法有效消除细胞内抗生素抗性基因 (i-ARG)，导致饮用水系统中普遍存在 ARG。_{在此，我们开发了在 Co 3} O ₄纳米线修饰电极上耦合电穿孔和电化学氧化的策略，以破坏多重抗性大肠杆菌细胞并促进后续的 i-ARG（bla _TEM-1和aac(3)-II）降解。纳米线尖端上的避雷针效应可以形成具有局部增强电场和高度集中电荷密度的有限区域，进而促进 ARB 细胞损伤的电穿孔和活性氯/氧物质生成的电化学反应。ARB 膜完整性和形态的表征表明，电穿孔诱导的细胞孔通过活性物质的氧化进一步扩大，导致在较低的施加电压下去除 i-ARG，并且能耗比传统电化学氧化方法低 6-9 倍与 Co ₃ O ₄-薄膜修饰电极。自来水中水平基因转移的令人满意的应用和有效抑制进一步证明了我们的策略在控制饮用水系统中 ARG 传播风险方面的巨大潜力。

"点击查看英文标题和摘要"

Lightning-Rod Effect on Nanowire Tips Reinforces Electroporation and Electrochemical Oxidation: An Efficient Strategy for Eliminating Intracellular Antibiotic Resistance Genes

Conventional oxidative disinfection methods are usually inefficient to eliminate intracellular antibiotic resistance genes (i-ARGs) due to competitive oxidation of cellular components of antibiotic-resistant bacteria (ARB), resulting in the ubiquitous occurrence of ARGs in drinking water systems. Herein, we developed the strategy of coupling electroporation and electrochemical oxidation on a Co₃O₄-nanowires-modified electrode to destroy the multiresistant Escherichia coli cells and promote subsequent i-ARG (bla_TEM-1 and aac(3)-II) degradation. The lightning-rod effect over nanowire tips can form finite regions with a locally enhanced electric field and highly concentrated charge density, in turn facilitating the electroporation for ARB cell damage and electrochemical reactivity for reactive chlorine/oxygen species generation. Characterization of the ARB membrane integrity and morphology revealed that electroporation-induced cell pores were further enlarged by the oxidation of reactive species, resulting in i-ARG removal at lower applied voltages and with 6–9 times lower energy consumption than the conventional electrochemical oxidation approach with a Co₃O₄-film-modified electrode. The satisfactory application and effective inhibition of horizontal gene transfer in tap water further demonstrated the great potential of our strategy in the control of the ARG dissemination risk in drinking water systems.

更新日期：2023-01-30

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南