Isolation of heavy metals-resistant bacteria from their original habitat is a crucial step in bioremediation. Six lead (Pb) resistant bacterial strains were isolated and identified utilizing 16S rRNA to be Enterobacter ludwigii FACU 4, Shigella flexneri FACU, Microbacterium paraoxydans FACU, Klebsiella pneumoniae subsp. pneumonia FACU, Raoultella planticola FACU 3 and Staphylococcus xylosus FACU. It was determined that all these strains had their Minimum inhibitory concentration (MIC) to be 2500 ppm except R. planticola FACU 3 has a higher maximum tolerance concentration (MTC) up to 2700 ppm. We evaluated the survival of all six strains on lead stress, the efficiency of biosorption and lead uptake. It was found that R. planticola FACU 3 is the highest MTC and S. xylosus FACU was the lowest MTC in this evaluation. Therefore, transmission electron microscopy (TEM) confirmed the difference between the morphological responses of these two strains to lead stress. These findings led to explore more about the genome of R. planticola FACU 3 using illumine Miseq technology. Draft genome sequence analysis revealed the genome size of 5,648,460 bp and G + C content 55.8% and identified 5526 CDS, 75 tRNA and 4 rRNA. Sequencing technology facilitated the identification of about 47 genes related to resistance to many heavy metals including lead, arsenic, zinc, mercury, nickel, silver and chromium of R. planticola FACU 3 strain. Moreover, genome sequencing identified plant growth-promoting genes (PGPGs) including indole acetic acid (IAA) production, phosphate solubilization, phenazine production, trehalose metabolism and 4-hydroxybenzoate production genes and a lot of antibiotic-resistant genes.

从其原始栖息地分离重金属抗性细菌是生物修复的关键步骤。利用16S rRNA分离并鉴定了六株铅 (Pb) 耐药菌株，分别为路德维氏肠杆菌FACU 4、福氏志贺菌FACU、副氧化微杆菌FACU、肺炎克雷伯菌亚种。肺炎FACU、Raoultella planticola FACU 3 和Staphylococcus xylosus FACU。确定所有这些菌株的最低抑制浓度 (MIC) 为 2500 ppm，除了R. planticolaFACU 3 具有更高的最大耐受浓度 (MTC)，高达 2700 ppm。我们评估了所有六种菌株在铅胁迫下的存活率、生物吸附效率和铅摄取。发现R. planticola FACU 3 是最高的 MTC，而S. xylosus FACU 是该评估中最低的 MTC。因此，透射电子显微镜 (TEM) 证实了这两种菌株对铅应力的形态反应之间的差异。这些发现导致使用 illumine Miseq 技术探索更多关于R. planticola FACU 3 基因组的信息。草图基因组序列分析显示基因组大小为 5,648,460 bp，G + C 含量为 55.8%，并鉴定出 5526 个 CDS、75个tRNA和 4 个rRNA. 测序技术有助于鉴定与R. planticola FACU 3 菌株的铅、砷、锌、汞、镍、银和铬等多种重金属抗性相关的约 47 个基因。此外，基因组测序鉴定出植物促生长基因（PGPGs），包括吲哚乙酸（IAA）产生、磷酸盐增溶、吩嗪产生、海藻糖代谢和4-羟基苯甲酸产生基因以及大量抗生素抗性基因。