恶性和良性乳腺疾病患者乳腺细胞的核变形能力分析,ACS Biomaterials Science & Engineering

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恶性和良性乳腺疾病患者乳腺细胞的核变形能力分析
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-01-27 , DOI: 10.1021/acsbiomaterials.2c01059
Ezgi Antmen ₁ , Menekse Ermis ₁ , Ozgur Kuren ₁ , Kemal Beksac ₂ , Cigdem Irkkan ₃ , Vasif Hasirci _{1,

4,

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Affiliation

乳腺癌是一种异质性和动态疾病，其中癌细胞对微环境的改变高度敏感。今天，传统的癌症检测方法给出了疾病状况的相当静态的图像，因此难以捕捉侵袭性和转移等动态特性。在这项研究中，将对乳腺癌患者进行的常规分子水平评估与使细胞变形的微图案化聚甲基丙烯酸甲酯 (PMMA) 生物材料表面的体外方法相结合。研究了细胞核的变形能力与癌症干性、侵袭性和转移之间的相关性。临床患者样本来自与肿瘤不同距离的乳房区域。使用 CD44/24、上皮细胞粘附标记 (EpCAM)、MUC1 和 PCK 研究了单细胞水平对微图案化表面的反应。结果表明，分子标记和形状描述符可以区分来自不同距离肿瘤中心和来自不同患者的细胞。具有最多转移性和侵袭性的细胞显示出最高的变形能力和最高水平的转移标记。总之，通过结合使用分子标记和核变形，可以在单细胞水平上改进异质性乳腺癌标本中亚群的检测和分离。结果表明，分子标记和形状描述符可以区分来自不同距离肿瘤中心和来自不同患者的细胞。具有最多转移性和侵袭性的细胞显示出最高的变形能力和最高水平的转移标记。总之，通过结合使用分子标记和核变形，可以在单细胞水平上改进异质性乳腺癌标本中亚群的检测和分离。结果表明，分子标记和形状描述符可以区分来自不同距离肿瘤中心和来自不同患者的细胞。具有最多转移性和侵袭性的细胞显示出最高的变形能力和最高水平的转移标记。总之，通过结合使用分子标记和核变形，可以在单细胞水平上改进异质性乳腺癌标本中亚群的检测和分离。

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Nuclear Deformability of Breast Cells Analyzed from Patients with Malignant and Benign Breast Diseases

Breast cancer is a heterogeneous and dynamic disease, in which cancer cells are highly responsive to alterations in the microenvironment. Today, conventional methods of detecting cancer give a rather static image of the condition of the disease, so dynamic properties such as invasiveness and metastasis are difficult to capture. In this study, conventional molecular-level evaluations of the patients with breast adenocarcinoma were combined with in vitro methods on micropatterned poly(methyl methacrylate) (PMMA) biomaterial surfaces that deform cells. A correlation between deformability of the nuclei and cancer stemness, invasiveness, and metastasis was sought. Clinical patient samples were from regions of the breast with different proximities to the tumor. Responses at the single-cell level toward the micropatterned surfaces were studied using CD44/24, epithelial cell adhesion marker (EpCAM), MUC1, and PCK. Results showed that molecular markers and shape descriptors can discriminate the cells from different proximities to the tumor center and from different patients. The cells with the most metastatic and invasive properties showed both the highest deformability and the highest level of metastatic markers. In conclusion, by using a combination of molecular markers together with nuclear deformation, it is possible to improve detection and separation of subpopulations in heterogenous breast cancer specimens at the single-cell level.

更新日期：2023-01-27

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