Probiotic-Functionalized Silk Fibroin/Sodium Alginate Scaffolds with Endoplasmic Reticulum Stress-Relieving Properties for Promoted Scarless Wound Healing,ACS Applied Materials & Interfaces

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Probiotic-Functionalized Silk Fibroin/Sodium Alginate Scaffolds with Endoplasmic Reticulum Stress-Relieving Properties for Promoted Scarless Wound Healing
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-01-26 , DOI: 10.1021/acsami.2c17168
Zhaona Dou ₁ , Binbin Li ₁ , Lin Wu ₂ , Tong Qiu ₁ , Xinyu Wang ₁ , Xueqiong Zhang ₃ , Ying Shen ₁ , Mengli Lu ₃ , Yan Yang ₁

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Bioactive substances such as probiotics are becoming a research hotspot in the field of tissue regeneration due to their excellent regulatory functions. Here, we proposed to load Lactobacillus casei onto a bilayer silk fibroin/sodium alginate (SF/SA) scaffold to endow the scaffold with both antibacterial and regenerative properties. The performance of the scaffold was characterized systemically. The L. casei-loaded scaffolds (L-SF/SA) bring in lactic acid, which has antibacterial and wound healing properties. In vitro, the cell-free supernatant (CFS) of L. casei inhibited the transformation of fibroblasts to myofibroblasts and relieved the endoplasmic reticulum stress (ERS). In vivo, L-SF/SA accelerated the healing of infected wounds in SD rats. The L-SF/SA reduced the bacterial load, induced M2 polarization of macrophages, increased angiogenesis, regulated collagen ratio, and alleviated the ERS, thereby promoting scarless wound healing and increasing hair follicle regeneration. Therefore, probiotic-functionalized silk fibroin/alginate scaffolds showed potential in the infected wound healing.

中文翻译：

益生菌功能化丝素蛋白/海藻酸钠支架具有内质网应力消除特性，可促进无疤痕伤口愈合

益生菌等生物活性物质以其优异的调节功能正成为组织再生领域的研究热点。在这里，我们建议将干酪乳杆菌加载到双层丝素蛋白/海藻酸钠 (SF/SA) 支架上，以赋予支架抗菌和再生特性。系统地表征了支架的性能。装载干酪乳杆菌的支架(L-SF/SA) 引入具有抗菌和伤口愈合特性的乳酸。在体外，干酪乳杆菌的无细胞上清液 (CFS)抑制了成纤维细胞向肌成纤维细胞的转化，并减轻了内质网应激 (ERS)。体内, L-SF/SA 加速了 SD 大鼠感染伤口的愈合。L-SF/SA 减少细菌负荷，诱导巨噬细胞 M2 极化，增加血管生成，调节胶原蛋白比例，减轻 ERS，从而促进无疤痕伤口愈合和增加毛囊再生。因此，益生菌功能化丝素蛋白/藻酸盐支架在感染伤口愈合中显示出潜力。

更新日期：2023-01-26

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