Abnormal levels of endogenous sulfur dioxide in organisms will cause cardiovascular, respiratory and neurological diseases. Therefore, the development of an effective detecting method for real-time, quantitative detection of sulfur dioxide derivatives in organisms is of great importance in physiological and pathological aspects. In this work, a red-emitting SO₂ fluorescent probe based on benzopyrylium derivatives were designed and synthesized to specifically detect SO₂ derivatives by nucleophilic addition of unsaturated carbon-carbon double bonds. The probe is characterized with rapid response, “naked eye” visualization, broad detection range and high sensitivity (8.1 μM) for SO₂ derivatives. In addition, it has been applied to track the dynamic, real-time, visual detection of exogenous and endogenous SO₂ in cells, and imaging of exogenous SO₂ derivatives in zebrafish.

生物体中内源性二氧化硫含量异常会导致心血管、呼吸系统和神经系统疾病。因此，开发一种实时、定量检测生物体内二氧化硫衍生物的有效检测方法，在生理和病理方面具有重要意义。本工作设计并合成了一种基于苯并吡喃衍生物的红光SO ₂荧光探针，通过不饱和碳-碳双键的亲核加成特异性检测SO _{2衍生物。}该探针具有响应速度快、“肉眼”可视化、检测范围广、SO _{2灵敏度高（8.1 μM）等特点}衍生品。_{此外，它还应用于细胞内外源性和内源性SO 2}的动态、实时、可视化检测，以及斑马鱼外源性SO ₂衍生物的成像。