Schizochytrium sp. 的聚酮合酶结构域的功能。解脂耶氏酵母脂肪酸合成研究,Journal of Agricultural and Food Chemistry

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Schizochytrium sp. 的聚酮合酶结构域的功能。解脂耶氏酵母脂肪酸合成研究
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-01-25 , DOI: 10.1021/acs.jafc.2c08383
Yu-Lei Jia ₁ , Fei Du ₁ , Fang-Tong Nong ₁ , Jin Li ₁ , Peng-Wei Huang ₁ , Wang Ma ₁ , Yang Gu ₁ , Xiao-Man Sun ₁

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众所周知，Schizochytrium sp。中的多不饱和脂肪酸（PUFA）。主要通过聚酮合酶（PKS）途径合成。然而，PKS参与脂肪酸合成的具体机制尚不清楚。在这项工作中，通过在解脂耶氏酵母中异源表达，研究了 ORFA、ORFB、ORFC 及其各个功能域基因在脂肪酸合成中的功能. 结果表明，ORFA、ORFB、ORFC及其各自功能域的表达均导致极长链PUFA（主要是二十碳五烯酸）含量的增加。此外，转录组学分析表明，除了 ORFB 的 3-酮脂酰-ACP 合酶 (KS) 结构域外，单个功能结构域的表达，包括丙二酰辅酶 A：ACP 酰基转移酶、3-羟酰基-ACP 脱水酶 (DH)、3 -酮脂酰-ACP 还原酶和 ORFA 的 KS 结构域、ORFB 的酰基转移酶结构域和 ORFC 的两个 DH 结构域导致三羧酸循环和磷酸戊糖途径的上调，三酰甘油生物合成、脂肪酸合成途径和 β-解脂耶氏酵母的氧化. 这些结果为揭示PKS在解脂耶氏酵母中脂肪酸合成中的作用和阐明PUFA生物合成的可能机制提供了理论依据。

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Function of the Polyketide Synthase Domains of Schizochytrium sp. on Fatty Acid Synthesis in Yarrowia lipolytica

It is well known that polyunsaturated fatty acids (PUFAs) in Schizochytrium sp. are mainly synthesized via the polyketide synthase (PKS) pathway. However, the specific mechanism of PKS in fatty acid synthesis is still unclear. In this work, the functions of ORFA, ORFB, ORFC, and their individual functional domain genes on fatty acid synthesis were investigated through heterologous expression in Yarrowia lipolytica. The results showed that the expression of ORFA, ORFB, ORFC, and their individual functional domains all led to the increase of the very long-chain PUFA content (mainly eicosapentaenoic acid). Furthermore, the transcriptomic analysis showed that except for the 3-ketoacyl-ACP synthase (KS) domain of ORFB, the expression of an individual functional domain, including malonyl-CoA: ACP acyltransferase, 3-hydroxyacyl-ACP dehydratase (DH), 3-ketoacyl-ACP reductase, and KS domains of ORFA, acyltransferase domains of ORFB, and two DH domains of ORFC resulted in upregulation of the tricarboxylic acid cycle and pentose phosphate pathway, downregulation of the triacylglycerol biosynthesis, fatty acid synthesis pathway, and β-oxidation in Yarrowia lipolytica. These results provide a theoretical basis for revealing the function of PKS in fatty acid synthesis in Y. lipolytica and elucidate the possible mechanism for PUFA biosynthesis.

更新日期：2023-01-25

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