基于 HPLC 的铁皮石斛代谢组学揭示其抗氧化能力,Frontiers in Plant Science

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基于 HPLC 的铁皮石斛代谢组学揭示其抗氧化能力
Frontiers in Plant Science ( IF 4.1 ) Pub Date : 2023-01-24 , DOI: 10.3389/fpls.2023.1060242
Zhengfei Luo ₁ , Lian Liu ₁ , Qiong Nie ₁ , Mingjin Huang ₁ , Chunlii Luo ₁ , Yedong Sun ₂ , Yongyan Ma ₂ , Jianxin Yu ₃ , Fuqiang Du ₃

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铁皮石斛是一种具有药用和营养特性的兰花，因其对健康的益处而受到越来越多的关注；然而，关于这些特性的代谢基础的信息有限。在这份报告中，次级代谢产物和抗氧化活性D. officinale使用基于 UHPLC-QqQ-MS/MS 的代谢组学方法分析了来自三个来源的茎样本。总共鉴定出411种代谢物，包括类黄酮和酚酸等8类，其中136种为差异代谢物。这些差异积累的代谢物（DAMs）主要富集在黄酮、黄酮醇、托烷、哌啶、吡啶、异喹啉生物碱生物合成和酪氨酸代谢等次级代谢途径中。代谢组学分析表明，类黄酮化合物的数量和含量占总代谢物的比例最高。层次聚类分析 (HCA) 表明，标记代谢物D. officinale三个来源的主要成分为黄酮类、生物碱类和酚酸类。相关性分析发现，48种差异代谢物与抗氧化能力呈显着正相关（r³0.8和p <0.0092），黄酮类化合物是影响不同抗氧化活性的主要因素。值得注意的是，quercetin-3-O-sophoroside-7-O-rhamnoside 和 dihydropinosylvin methyl ether 可能是造成云南种源（YN）、浙江种源（ZJ）和贵州种源抗氧化能力差异的主要化合物（广州）。这些发现为筛选品种、质量控制和产品开发提供了有价值的信息。D. officinale.

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HPLC-based metabolomics of Dendrobium officinale revealing its antioxidant ability

Dendrobium officinale is an orchid with medicinal and nutritional properties that has received increasing attention because of its health benefits; however, there is limited information about the metabolic basis of these properties. In this report, secondary metabolites and the antioxidant activity of D. officinale stem samples from three provenances were analyzed, using a UHPLC-QqQ-MS/MS-based metabolomics approach. In total, 411 metabolites were identified including 8 categories such as flavonoids and phenolic acids, 136 of which were differential metabolites. These differentially accumulated metabolites (DAMs) were mainly enriched in secondary metabolic pathways such as flavone, flavonol, tropane, piperidine, pyridine, isoquinoline alkaloid biosynthesis and tyrosine metabolism. The metabolomic profiling suggested that the quantity and content of flavonoid compounds accounted for the highest proportion of total metabolites. Hierarchical cluster analysis (HCA) showed that the marker metabolites of D. officinale from the three provenances were mainly flavonoids, alkaloids and phenolic acids. Correlation analysis identified that 48 differential metabolites showed a significant positive correlation with antioxidant capacity (r ³ 0.8 and p < 0.0092), and flavonoids were the main factors affecting the different antioxidant activities. It is worth noting that quercetin-3-O-sophoroside-7-O-rhamnoside and dihydropinosylvin methyl ether might be the main compounds causing the differences in antioxidant capacity of Yunnan provenance (YN), Zhejiang provenance (ZJ), and Guizhou provenance (GZ). These finding provides valuable information for screening varieties, quality control and product development of D. officinale.

更新日期：2023-01-24

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