被子植物脂氧合酶 (LOX) 基因的全基因组分析。,Plants

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被子植物脂氧合酶 (LOX) 基因的全基因组分析。
Plants ( IF 4.0 ) Pub Date : 2023-01-14 , DOI: 10.3390/plants12020398
Paula Oliveira Camargo ₁ , Natália Fermino Calzado ₁ , Ilara Gabriela Frasson Budzinski _{1,

2} , Douglas Silva Domingues _{1,

2}

Affiliation

脂氧合酶 (LOX) 是催化氧分子加成不饱和脂肪酸从而形成氢过氧化物的酶。在植物中，这些酶由一个多基因家族编码，该家族存在于多个具有不同活动模式的器官中，它们被归类为 LOX9 或 LOX13。它们参与多种生理功能，例如生长、果实发育和植物防御。尽管对植物中 LOX 家族的基因进行了多项研究，但大多数研究仅限于单一物种或少数密切相关的物种。本研究旨在分析被子植物 LOX 基因的多样性、进化和表达。我们在 23 种被子植物和基础植物中鉴定了 247 个 LOX 基因。系统发育分析确定了支持 LOX13 的进化枝和 LOX9 的两个主要进化枝：LOX9_A 和 LOX9_B。真双子叶植物如 Tarenaya hassleriana、Capsella rubella 和 Arabidopsis thaliana 不存在 LOX9_B 基因；然而，LOX9_B 存在于本研究中使用的所有单子叶植物中。我们发现 LOX9 和 LOX13 存在潜在的新亚细胞定位模式和保守的氧化残基尚未探索。总之，我们的研究为被子植物脂肪氧化酶的进一步功能和进化研究提供了基础。

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Genome-Wide Analysis of Lipoxygenase (LOX) Genes in Angiosperms.

Lipoxygenases (LOXs) are enzymes that catalyze the addition of an oxygen molecule to unsaturated fatty acids, thus forming hydroperoxides. In plants, these enzymes are encoded by a multigene family found in several organs with varying activity patterns, by which they are classified as LOX9 or LOX13. They are involved in several physiological functions, such as growth, fruit development, and plant defense. Despite several studies on genes of the LOX family in plants, most studies are restricted to a single species or a few closely related species. This study aimed to analyze the diversity, evolution, and expression of LOX genes in angiosperm species. We identified 247 LOX genes among 23 species of angiosperms and basal plants. Phylogenetic analyses identified clades supporting LOX13 and two main clades for LOX9: LOX9_A and LOX9_B. Eudicot species such as Tarenaya hassleriana, Capsella rubella, and Arabidopsis thaliana did not present LOX9_B genes; however, LOX9_B was present in all monocots used in this study. We identified that there were potential new subcellular localization patterns and conserved residues of oxidation for LOX9 and LOX13 yet unexplored. In summary, our study provides a basis for the further functional and evolutionary study of lipoxygenases in angiosperms.

更新日期：2023-01-14

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