用于 Mucin-1 电化学检测的功能化噻吩基 Aptasensors,ACS Applied Polymer Materials

当前位置： X-MOL 学术 › ACS Appl. Polym. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于 Mucin-1 电化学检测的功能化噻吩基 Aptasensors
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2023-01-20 , DOI: 10.1021/acsapm.2c01739
Grace Haya ₁ , Damilola O. Runsewe ₂ , Mackenzie U. Otakpor ₁ , Gabriel E. Pohlman ₁ , Adelyne Towne ₁ , Tania Betancourt _{1,

2} , Jennifer A. Irvin _{1,

2}

Affiliation

Mucin-1 (MUC1) 是一种在上皮组织中发现的糖蛋白；它的功能是通过阻止病原体到达细胞来保护身体。在乳腺癌、肺癌、胃癌、卵巢癌和许多其他类型的恶性肿瘤中观察到这种蛋白质的过度表达和升高的血清水平。目前用于检测癌症的方法非常昂贵，因此不易获得；有些方法也是侵入性的。检测 MUC1 的能力可以实现癌症的早期检测，从而获得更成功的结果。本研究的重点是开发基于适体功能化电活性聚合物 (EAP) 的稳健生物传感器平台，该平台可用于检测癌症。为实现这一目标，对氧化铟锡滑动表面进行了改性，以实现 3 的电活性共聚物的电化学生长，H-噻吩并[3,4- b ][1,4]二氧杂环庚烷-3,3-二基)二乙酸 (ProDOT(COOH) ₂ )，添加了羧酸官能团以引入 MUC1 特异性适体的键合位点。研究了三种共聚物比例以最大化性能。然后将适体连接到 EAP 以创建可用于 MUC1 多肽电化学检测的适体传感器。评估了生物传感器的检测限及其稳定性。当储存在 0.1 M 磷酸盐缓冲盐水中时，MUC1 适体传感器显示稳定性至少 6 天，具体取决于共聚物的比例。1:2 EDOT/ProDOT(COOH) ₂发现共聚物随着时间的推移最稳定，并提供最小的检测限之一，使其成为适体传感器优化的最有利比例。具体而言，1:2 EDOT/ProDOT(COOH) ₂生物传感器提供了 369 fg/mL (418 fM) 的检测限和 625 fg/mL 至 6.25 ng/mL（709 fM 至 7.09 nM）的线性范围MUC1 肽 APDTRPAPG。当与包括 IgG 和细胞培养基在内的竞争试剂进行测试时，该传感器还显示出选择性。aptasensor 的性能证明了它作为用于 MUC1 检测的高灵敏度和选择性生物传感器的潜力。

"点击查看英文标题和摘要"

Functionalized Thiophene-Based Aptasensors for the Electrochemical Detection of Mucin-1

Mucin-1 (MUC1) is a glycoprotein found in epithelial tissues; its function is to protect the body by blocking pathogens from reaching the cells. Overexpression and elevated serum levels of this protein are observed in breast cancer, lung cancer, stomach cancer, ovarian cancer, and many other types of malignancies. Current methods used to detect cancer are expensive and therefore not readily accessible; some methods are also invasive. The ability to detect MUC1 could allow for early detection of cancer, leading to more successful outcomes. This research focuses on the development of a robust biosensor platform based on aptamer-functionalized electroactive polymers (EAPs) that can be used for the detection of cancer. To achieve this, indium tin oxide slide surfaces were modified to enable the electrochemical growth of an electroactive copolymer of 3,4-ethylenedioxythiophene (EDOT) and 2,2-(3,4-dihydro-2H-thieno[3,4-b][1,4]dioxepine-3,3-diyl)diacetic acid (ProDOT(COOH)₂), with the carboxylic acid functionalities added to introduce bonding sites for a MUC1-specific aptamer. Three copolymer ratios were investigated to maximize the performance. The aptamer was then attached to the EAPs to create aptasensors that could be used for the electrochemical detection of a MUC1 polypeptide. The limits of detection of the biosensors and their stabilities were evaluated. The MUC1 aptasensor showed stability for at least 6 days, depending on the ratio of the copolymer, when stored in 0.1 M phosphate-buffered saline. The 1:2 EDOT/ProDOT(COOH)₂ copolymer was found to be the most stable over time and to offer one of the smallest limits of detection, making it the most favorable ratio for aptasensor optimization. Specifically, the 1:2 EDOT/ProDOT(COOH)₂ biosensor provided a limit of detection of 369 fg/mL (418 fM) and a linear range of 625 fg/mL to 6.25 ng/mL (709 fM to 7.09 nM) with the MUC1 peptide APDTRPAPG. The sensor also showed selectivity when tested with competing agents including IgG and cell media. The performance of the aptasensor demonstrated its potential as a highly sensitive and selective biosensor for MUC1 detection.

更新日期：2023-01-20

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南