This study investigated the ability of activated sludge (AS) to biodegrade triisobutyl phosphate (TiBP) after acclimation in an AS bioreactor by adding 50 mg/L TiBP. The bioreactor significantly increased the biotransformation rate of TiBP (2.15–12.7 d⁻¹) over two months of acclimation. Seven transformation products (TPs) of TiBP were identified by high-resolution mass spectrometry, and hydrolysis, hydroxylation and dehydrogenation were the major biodegradation pathways of TiBP. TiBP degradation solutions at 0, 3, 7, and 10 h showed significantly toxic effects on zebrafish embryos, while the toxicity of TiBP degradation solutions at 24 h significantly decreased. Pseudomonas was inferred to be a specific bacterial population in the TiBP metabolic microbial consortium (TMMC) that degrades TiBP (p < 0.001). When TMMC (0.5, 1, and 2 g_ss/L) was introduced into AS, the TiBP biotransformation rates (1.97, 2.05, and 2.26 d⁻¹ at 1.0 mg/L TiBP, and 0.09, 0.11, and 0.83 d⁻¹ at 30.0 mg/L TiBP) were significantly enhanced compared to the control (0.31 and 0.07 d⁻¹) without TMMC inoculation. In general, this study provides new insights into the key species populations that accelerate TiBP degradation and promote the development of TiBP reduction biotechnology in WWTPs.

本研究通过添加 50 mg/L TiBP，调查了在 AS 生物反应器中驯化后活性污泥 (AS) 生物降解磷酸三异丁酯 (TiBP) 的能力。经过两个月的适应后，生物反应器显着提高了 TiBP 的生物转化率 (2.15–12.7 d ^{-1 )。}通过高分辨率质谱法鉴定了 TiBP 的七种转化产物 (TP)，水解、羟基化和脱氢是 TiBP 的主要生物降解途径。TiBP 降解溶液在 0、3、7 和 10 h 时对斑马鱼胚胎表现出显着的毒性作用，而 TiBP 降解溶液在 24 h 时的毒性显着降低。假单胞菌属被推断为 TiBP 代谢微生物联合体 (TMMC) 中的特定细菌种群，可降解 TiBP ( p  < 0.001)。当 TMMC（0.5、1 和 2 g _ss /L）被引入 AS 时，TiBP 生物转化率（1.97、2.05 和 2.26 d ^-1在 1.0 mg/L TiBP 和 0.09、0.11 和 0.83 d ^-1与未接种 TMMC 的对照（0.31 和 0.07 d ^-1 ）相比，在 30.0 mg/L TiBP 下）显着增强。总的来说，这项研究为加速 TiBP 降解和促进污水处理厂 TiBP 还原生物技术发展的关键物种种群提供了新的见解。