水稻和根际微生物组对有机磷酸酯的生物转化：多代谢途径、机制和毒性评估,Environmental Science & Technology

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水稻和根际微生物组对有机磷酸酯的生物转化：多代谢途径、机制和毒性评估
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-01-19 , DOI: 10.1021/acs.est.2c07796
Yuanyuan Yu ₁ , Xiaolong Yu ₁ , Dongqing Zhang ₁ , Ling Jin _{2,

3} , Jiahui Huang ₁ , Xifen Zhu ₁ , Jianteng Sun ₁ , Miao Yu ₄ , Lizhong Zhu ₅

Affiliation

通过水培实验全面研究了有机磷酸酯 (OPE) 在水稻和根际微生物组中的生物转化行为和毒性。疏水性较低的 OPEs 容易在根际转运，根际微生物组可以减少 OPEs 在水稻组织中的摄取和转运。在水稻和根际微生物组中成功鉴定出新的代谢物，包括水解、羟基化、甲基化以及谷胱甘肽、葡萄糖醛酸和硫酸盐结合产物。根际细菌和植物可以合作形成一个复杂的生态相互作用网络来消除 OPE。此外，揭示了 OPE 降解过程中根际微生物组的活跃成员，宏基因组分析表明，这些活跃种群中的大多数都含有 OPE 降解基因。用于植物毒性评估的代谢组学分析结果表明，发现水稻植物的几个关键功能代谢途径受到代谢物的干扰，例如磷酸二苯酯和磷酸一苯酯。此外，涉及的代谢机制，如碳水化合物代谢、氨基酸代谢与合成、核苷酸代谢等。大肠杆菌在暴露于根际微生物群产生的 OPE 产物混合物后发生了显着变化。这项工作首次全面了解了 OPE 在植物和相关微生物组中的整个代谢过程，并为正在进行的新兴污染物及其转化产物的风险评估提供了支持。

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Biotransformation of Organophosphate Esters by Rice and Rhizosphere Microbiome: Multiple Metabolic Pathways, Mechanism, and Toxicity Assessment

The biotransformation behavior and toxicity of organophosphate esters (OPEs) in rice and rhizosphere microbiomes were comprehensively studied by hydroponic experiments. OPEs with lower hydrophobicity were liable to be translocated acropetally, and rhizosphere microbiome could reduce the uptake and translocation of OPEs in rice tissues. New metabolites were successfully identified in rice and rhizosphere microbiome, including hydrolysis, hydroxylated, methylated, and glutathione-, glucuronide-, and sulfate-conjugated products. Rhizobacteria and plants could cooperate to form a complex ecological interaction web for OPE elimination. Furthermore, active members of the rhizosphere microbiome during OPE degradation were revealed and the metagenomic analysis indicated that most of these active populations contained OPE-degrading genes. The results of metabolomics analyses for phytotoxicity assessment implied that several key function metabolic pathways of the rice plant were found perturbed by metabolites, such as diphenyl phosphate and monophenyl phosphate. In addition, the involved metabolism mechanisms, such as the carbohydrate metabolism, amino acid metabolism and synthesis, and nucleotide metabolism in Escherichia coli, were significantly altered after exposure to the products mixture of OPEs generated by rhizosphere microbiome. This work for the first time gives a comprehensive understanding of the entire metabolism of OPEs in plants and associated microbiome, and provides support for the ongoing risk assessment of emerging contaminants and, most critically, their transformation products.

更新日期：2023-01-19

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