Diisobutyl phthalate (DIBP), as a plasticizer, is widely used and has caused many extreme soil contamination scenarios, posing potential risks to soil fauna. However, the toxic effects and mechanisms of DIBP on soil fauna remain unclear. In this study, earthworms () were used as model animals to explore the subchronic toxicity of extreme DIBP soil exposure (300, 600, and 1200 mg/kg) for 28 days. The results showed that the level of reactive oxygen species (ROS) and the contents of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) in were significantly increased during continuous DIBP exposure. In addition, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were significantly inhibited while glutathione S-transferase (GST) activity was activated during continuous exposure. Integrated biological response (IBR) analysis showed that DIBP had positive dose-dependent toxicity and negative time-dependent toxicity to , and SOD/CAT were selected as sensitive biomarkers. The molecular docking study found that DIBP could stably bind to SOD/CAT through hydrogen bonding, which further proved its sensitivity. This study provides primary data for ecological and environmental risk assessment of extreme dose DIBP soil pollution.

中文翻译：

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极端环境剂量的邻苯二甲酸二异丁酯暴露会引起蚯蚓（Eisenia fetida）的氧化应激和 DNA 损伤：生化和分子水平的证据


邻苯二甲酸二异丁酯（DIBP）作为增塑剂的广泛使用，造成了许多极端的土壤污染场景，对土壤动物构成潜在风险。然而，DIBP对土壤动物的毒性作用和机制仍不清楚。在这项研究中，以蚯蚓 () 作为模型动物，探讨极端 DIBP 土壤暴露（300、600 和 1200 mg/kg）28 天的亚慢性毒性。结果表明，持续暴露于DIBP期间，活性氧（ROS）水平以及丙二醛（MDA）和8-羟基脱氧鸟苷（8-OHdG）含量显着增加。此外，连续暴露期间超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性显着受到抑制，而谷胱甘肽S-转移酶（GST）的活性被激活。综合生物反应（IBR）分析表明，DIBP 对 DIBP 具有正向剂量依赖性毒性和负向时间依赖性毒性，并选择 SOD/CAT 作为敏感生物标志物。分子对接研究发现DIBP能够通过氢键稳定地与SOD/CAT结合，进一步证明了其敏感性。本研究为极端剂量DIBP土壤污染生态环境风险评估提供了原始数据。