<br>降解的 TBBPA（二溴双酚 A）通过凋亡和坏死途径具有高细胞毒性,Heliyon

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降解的 TBBPA（二溴双酚 A）通过凋亡和坏死途径具有高细胞毒性
Heliyon ( IF 3.4 ) Pub Date : 2023-01-16 , DOI: 10.1016/j.heliyon.2023.e13003
Keitaro Suyama ₁ , Hitoshi Kesamaru ₂ , Takashi Okubo ₂ , Kazumi Kasatani ₂ , Keisuke Tomohara ₁ , Ayami Matsushima ₂ , Takeru Nose _{1,

2}

Affiliation

已经研究了包含双酚 A （BPA）衍生物的卤化阻燃剂，例如四溴双酚 A （TBBPA），它们对人类健康的不利影响。然而，尽管这些卤化 BPA 很容易在环境中降解，但这些降解产物对生物体的风险大多被忽视了。为了评估降解的 TBBPAs 和相关化合物的潜在毒性，我们在体外检查了具有 1 至 4 个卤素原子的卤代双酚 A 衍生物的细胞毒性。结果表明，降解的 TBBPA 衍生物对 HeLa 细胞的细胞毒性强于 TBBPA。有趣的是，在测试化合物中，具有两个卤原子的二卤代 BPA 衍生物表现出最强的细胞毒性。此外，乳酸脱氢酶释放试验、荧光光谱和流式细胞术结果表明，二溴 BPA 和二碘 BPA 通过破坏 HeLa 细胞的细胞膜诱导凋亡和坏死细胞死亡。此外，在脱卤 TBBPA 和相关化合物存在下，大肠杆菌的生长受到抑制。这些发现表明，从各种含阻燃剂的产品中泄漏的卤代 BPA 衍生物需要严格监测，因为不仅 TBBPA 及其在环境中的降解产品也会对人体健康产生不利影响。

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High cytotoxicity of a degraded TBBPA, dibromobisphenol A, through apoptotic and necrosis pathways

Halogenated flame retardants comprising bisphenol A (BPA) derivatives, such as tetrabromobisphenol A (TBBPA), have been studied their adverse effects on human health. However, despite the fact that these halogenated BPAs are easily degraded in the environment, the risks to living organisms due to these degraded products have mostly been overlooked. To evaluate the potential toxicity of degraded TBBPAs and related compounds, we examined the cytotoxicity of halogenated bisphenol A derivatives possessing one to four halogen atoms in vitro. The results indicated that the degraded TBBPA derivatives exhibited strong cytotoxicity against HeLa cells than TBBPA. Interestingly, the di-halogenated BPA derivatives possessing two halogen atoms exhibited the strongest cytotoxicity among tested compounds. In addition, a lactate dehydrogenase release assay, fluorescence spectroscopy and flow cytometry results indicated that dibromo-BPA and diiodo-BPA induced both apoptotic and necrotic cell death by damaging the cell membranes of HeLa cells. Moreover, Escherichia coli growth was inhibited in the presence of dehalogenated TBBPA and related compounds. These findings suggest that halogenated BPA derivatives that leak from various flame-retardant-containing products require strict monitoring, as not only TBBPA but also its degraded products in environment can exert adverse effects to human health.

更新日期：2023-01-16

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