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Liver-Targeted Near-Infrared Fluorescence/Photoacoustic Dual-Modal Probe for Real-Time Imaging of In Situ Hepatic Inflammation
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-01-15 , DOI: 10.1021/acs.analchem.2c05476 Yong Zhang 1 , Wei Li 2 , Xueqian Chen 1 , Shaoqing Xiong 1 , Yongning Bian 1 , Lin Yuan 2 , Xueyun Gao 1 , Dongdong Su 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-01-15 , DOI: 10.1021/acs.analchem.2c05476 Yong Zhang 1 , Wei Li 2 , Xueqian Chen 1 , Shaoqing Xiong 1 , Yongning Bian 1 , Lin Yuan 2 , Xueyun Gao 1 , Dongdong Su 1
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Early diagnosis of hepatic inflammation is the key to timely treatment and avoid the worsening of liver inflammation. Near-infrared fluorescence (NIRF) probes have high sensitivity but low spatial resolution in lesion imaging, while photoacoustic (PA) imaging has good spatial location information. Therefore, the development of a NIRF/PA dual-modal probe integrated with high sensitivity and spatial location feedback can achieve an accurate early diagnosis of hepatic inflammation. Here, we report an activatable NIRF/PA dual-modal probe (hCy-Tf-CA) for the detection of the superoxide anion (O2·–) in early hepatic inflammation. hCy-Tf-CA showed high selectivity and sensitivity for detecting O2·– fluctuation in vitro. More importantly, by introducing hepatocyte-targeting cholic acid (CA), the probe successfully achieved accurate in situ imaging of acute inflammatory liver injury (AILI) and autoimmune hepatitis (AIH) in vivo. The introduced CA not only promotes the hepatic targeting accumulation of probes but also improves the performance of low background dual-modal imaging in vivo. Therefore, hCy-Tf-CA provides an effective strategy for significantly improving in situ imaging performance and holds great potential for early, sensitive, and accurate diagnosis of hepatic inflammation.
中文翻译：
肝脏靶向近红外荧光/光声双模态探针，用于原位肝脏炎症的实时成像
肝脏炎症的早期诊断是及时治疗、避免肝脏炎症恶化的关键。近红外荧光（NIRF）探针在病变成像中灵敏度高但空间分辨率低，而光声（PA）成像具有良好的空间位置信息。因此，开发结合高灵敏度和空间位置反馈的NIRF/PA双模态探针可以实现肝脏炎症的准确早期诊断。在这里，我们报告了一种可激活的 NIRF/PA 双模式探针 ( hCy-Tf-CA )，用于检测早期肝脏炎症中的超氧阴离子 (O 2 ·– )。hCy-Tf-CA检测O 2 ·–波动具有高选择性和灵敏性体外。更重要的是，该探针通过引入靶向肝细胞的胆酸（CA ），成功实现了对急性炎症性肝损伤（AILI）和自身免疫性肝炎（AIH）的体内精确原位成像。引入的CA不仅促进了探针的肝脏靶向积累，而且提高了体内低背景双模态成像的性能。因此，hCy-Tf-CA为显着提高原位成像性能提供了一种有效策略，并在肝脏炎症的早期、灵敏和准确诊断方面具有巨大潜力。
更新日期：2023-01-15
中文翻译：
肝脏靶向近红外荧光/光声双模态探针，用于原位肝脏炎症的实时成像
肝脏炎症的早期诊断是及时治疗、避免肝脏炎症恶化的关键。近红外荧光（NIRF）探针在病变成像中灵敏度高但空间分辨率低，而光声（PA）成像具有良好的空间位置信息。因此，开发结合高灵敏度和空间位置反馈的NIRF/PA双模态探针可以实现肝脏炎症的准确早期诊断。在这里，我们报告了一种可激活的 NIRF/PA 双模式探针 ( hCy-Tf-CA )，用于检测早期肝脏炎症中的超氧阴离子 (O 2 ·– )。hCy-Tf-CA检测O 2 ·–波动具有高选择性和灵敏性体外。更重要的是，该探针通过引入靶向肝细胞的胆酸（CA ），成功实现了对急性炎症性肝损伤（AILI）和自身免疫性肝炎（AIH）的体内精确原位成像。引入的CA不仅促进了探针的肝脏靶向积累，而且提高了体内低背景双模态成像的性能。因此，hCy-Tf-CA为显着提高原位成像性能提供了一种有效策略，并在肝脏炎症的早期、灵敏和准确诊断方面具有巨大潜力。
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