用于骨再生的纤维外脱矿牙本质基质,Advanced Healthcare Materials

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用于骨再生的纤维外脱矿牙本质基质
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2023-01-14 , DOI: 10.1002/adhm.202202611
Xiaoyi Wu ₁ , Wenan Peng ₁ , Gufeng Liu ₁ , Shilei Wang ₂ , Bo Duan ₂ , Jian Yu ₁ , Hongye Yang ₁ , Cui Huang ₁

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牙本质是一种天然的细胞外基质，但由于缺乏适当的治疗，其在骨移植和组织工程应用中的可用性被低估。在这项研究中，纤维外脱矿的概念首次被引入用于骨再生的牙本质衍生生物材料的构建中。具有大分子量的钙螯合剂用于选择性地去除纤维外磷灰石矿物质而不干扰牙本质胶原内的纤维内矿物质，从而形成纤维外脱矿质牙本质基质（EDM）。发现具有独特纳米形貌和类骨机械特性的 EDM 在体外显着促进细胞粘附、迁移和成骨分化，同时增强大鼠颅骨缺损的体内骨愈合。EDM 出色的成骨性能进一步证实与粘着斑-细胞骨架-细胞核机械传导轴的激活有关。总的来说，这项研究表明，牙本质的纤维外脱矿具有生产用于骨再生的分层胶原基支架的巨大潜力，这种简便的自上而下的制造方法为天然来源的生物活性材料的生物医学应用带来了新思路。

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Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

Dentin is a natural extracellular matrix, but its availability in bone grafting and tissue engineering applications is underestimated due to a lack of proper treatment. In this study, the concept of extrafibrillar demineralization is introduced into the construction of dentin-derived biomaterials for bone regeneration for the first time. Calcium chelating agents with large molecular weights are used to selectively remove the extrafibrillar apatite minerals without disturbing the intrafibrillar minerals within dentin collagen, resulting in the formation of an extrafibrillarly demineralized dentin matrix (EDM). EDM with distinctive nanotopography and bone-like mechanical properties is found to significantly promote cell adhesion, migration, and osteogenic differentiation in vitro while enhancing in vivo bone healing of rat calvarial defects. The outstanding osteogenic performance of EDM is further confirmed to be related to the activation of the focal adhesion–cytoskeleton–nucleus mechanotransduction axis. Overall, this study shows that extrafibrillar demineralization of dentin has great potential to produce hierarchical collagen-based scaffolds for bone regeneration, and this facile top-down fabrication method brings about new ideas for the biomedical application of naturally derived bioactive materials.

更新日期：2023-01-14

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