The mitochondrial genome of Binodoxys acalephae (Hymenoptera: Braconidae) with unique gene rearrangement and phylogenetic implications,Molecular Biology Reports

当前位置： X-MOL 学术 › Mol. Biol. Rep. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The mitochondrial genome of Binodoxys acalephae (Hymenoptera: Braconidae) with unique gene rearrangement and phylogenetic implications
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2023-01-13 , DOI: 10.1007/s11033-022-08232-0
Shiwen Xu ₁ , Weiwei Li _{2,

3} , Qiannan Liu ₁ , Yunming Wang ₄ , Xiaoling Li ₄ , Xiaoqian Duan ₄ , Jia He _{5,

6} , Fan Song ₁

Affiliation

Background

Species in the subfamily Aphidiinae from the Braconidae of Hymenoptera are endoparasitic wasps that exclusively utilize aphids as hosts. Some Aphidiinae species are widely used as biological agents. However, there were only one species with determined complete mitochondrial genome from this subfamily.

Methods and results

In this study, we sequenced and annotated the mitochondrial genome (mitogenome) of Binodoxys acalephae, which was 15,116 bp in size and contained 37 genes. The start codon of 13 protein-coding genes was ATN, and the complete stop codon TAA and TAG was widely assigned to 11 protein-coding genes. The lrRNA contains 43 stem-loop structures, and srRNA contains 25 stem-loop structures. Translocation and inversion of tRNA genes was found to be dominant in B. acalephae. In contrast to Aphidius gifuensis from the same subfamily Aphidiinae, inverted tRNA^Leu1 was translocated to the gene cluster between tRNA^Leu2 and COX2, and the control region between tRNA^Ile and tRNA^Met was deleted in the mitogenome of B. acalephae. Within Braconidae, gene clusters tRNA^Trp-tRNA^Cys-tRNA^Tyr and CR-tRNA^Ile-tRNA^Gln-tRNA^Met were hotspots for gene rearrangement. Phylogenetic analysis showed that both Bayesian and maximum-likelihood methods recovered the monophyly of Aphidiinae and suggested that Aphidiinae formed sister clades with the remaining subfamilies. The phylogenetic analyses of nine subfamilies supported the monophyly of Cyclostomes and Noncyclostomes in Braconidae.

Conclusion

The arrangement of mitochondrial genes and the phylogenetic relationships among nine Braconidae subfamilies were constructed better to understand the diversity and evolution of Aphidiinae mitogenomes.

中文翻译：

Binodoxys acalephae（膜翅目：茧蜂科）的线粒体基因组具有独特的基因重排和系统发育意义

背景

膜翅目茧蜂科蚜亚科的物种是专门利用蚜虫作为宿主的内寄生黄蜂。一些蚜亚科物种被广泛用作生物制剂。然而，该亚科中只有一个物种具有完整的线粒体基因组。

方法和结果

在本研究中，我们对 Binodoxys acalephae的线粒体基因组（线粒体基因组）进行了测序和注释，该基因组大小为 15,116 bp，包含 37 个基因。 13个蛋白编码基因的起始密码子为ATN，完整终止密码子TAA和TAG被广泛分配给11个蛋白编码基因。 lrRNA包含43个茎环结构， srRNA包含25个茎环结构。发现 tRNA 基因的易位和倒位在B. acalephae中占主导地位。与来自同一亚科Aphidiinae的Aphidius gifuensis相比，倒转的tRNA ^Leu1被易位到tRNA ^Leu2和COX2之间的基因簇，并且tRNA ^Ile和tRNA ^Met之间的控制区域在B. acalephae的有丝分裂基因组中被删除。在茧蜂科中，基因簇tRNA ^Trp - tRNA ^Cys -tRNA ^Tyr和 CR- tRNA ^Ile -tRNA ^Gln -tRNA ^Met是基因重排的热点。系统发育分析表明，贝叶斯法和最大似然法均恢复了蚜亚科的单系性，并表明蚜亚科与其余亚科形成姐妹支系。九个亚科的系统发育分析支持了茧虫科中圆口目和非圆口目的单系性。