3-(3-Phenoxybenzyl)amino-β-carboline showed extremely-high activity; the IC value was 0.074μM. To verify -induced cell death types, we observed the chromatin condensation, the DNA fragmentation and activated caspase-3 using Hoechst 33342, agarose electrophoresis and western blot, and suggesting -induced cell death type was apoptosis. Flow cytometry showed that -treated cell was induced SubG1 cell population after G2/M cell cycle arrest. In addition, using affinity chromatography and peptide mass fingerprinting, we found that interacting protein with this compound was α-tubulin protein.

中文翻译：

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3-(3-苯氧基苄基)氨基-β-咔啉：一种靶向α-微管蛋白的新型抗肿瘤药物

3-(3-苯氧基苄基)氨基-β-咔啉表现出极高的活性；IC值为0.074μM。为了验证诱导的细胞死亡类型，我们使用Hoechst 33342、琼脂糖电泳和蛋白质印迹观察了染色质浓缩、DNA断裂和激活的caspase-3，并提示诱导的细胞死亡类型为凋亡。流式细胞术显示经处理的细胞在G2/M细胞周期停滞后被诱导为SubG1细胞群。此外，利用亲和色谱和肽质量指纹图谱分析，我们发现与该化合物相互作用的蛋白是α-微管蛋白。