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Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-01-10 , DOI: 10.1021/jacs.2c12567 Ilya Likhotkin 1 , Richard Lincoln 1 , Mariano L Bossi 2 , Alexey N Butkevich 1, 2 , Stefan W Hell 1, 2
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-01-10 , DOI: 10.1021/jacs.2c12567 Ilya Likhotkin 1 , Richard Lincoln 1 , Mariano L Bossi 2 , Alexey N Butkevich 1, 2 , Stefan W Hell 1, 2
Affiliation
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We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells, and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of photoactivatable labels for strain-promoted tetrazine ligation and self-labeling protein tags (HaloTag, SNAP-tag), and duplexing of an imaging channel with another large Stokes shift dye have been demonstrated.
中文翻译：
用于多色纳米显微镜的光活化大斯托克斯位移荧光团
我们基于 1-vinyl-10-silaxanthone 亚胺核心结构设计了具有红色荧光发射和~100 nm Stokes 位移的无笼化基团光活化活细胞渗透染料。拟议的荧光团经过无副产物的单光子和双光子激活，适用于固定细胞和活细胞的多色荧光显微术，并且与 STED（受激发射耗尽）和 PALM（光激活定位显微术）等超分辨率技术兼容. 使用可光激活标签进行应变促进的四嗪连接和自标记蛋白质标签（HaloTag、SNAP 标签），以及使用另一种大型 Stokes 位移染料对成像通道进行双工处理。
更新日期：2023-01-10
中文翻译：
用于多色纳米显微镜的光活化大斯托克斯位移荧光团
我们基于 1-vinyl-10-silaxanthone 亚胺核心结构设计了具有红色荧光发射和~100 nm Stokes 位移的无笼化基团光活化活细胞渗透染料。拟议的荧光团经过无副产物的单光子和双光子激活，适用于固定细胞和活细胞的多色荧光显微术，并且与 STED（受激发射耗尽）和 PALM（光激活定位显微术）等超分辨率技术兼容. 使用可光激活标签进行应变促进的四嗪连接和自标记蛋白质标签（HaloTag、SNAP 标签），以及使用另一种大型 Stokes 位移染料对成像通道进行双工处理。
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