高强度聚焦超声结合 Ti3C2–TiO2 增强鲁米诺的电化学发光，用于多核苷酸激酶的灵敏检测,ACS Applied Materials & Interfaces

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高强度聚焦超声结合 Ti3C2–TiO2 增强鲁米诺的电化学发光，用于多核苷酸激酶的灵敏检测
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-01-11 , DOI: 10.1021/acsami.2c19539
Zhihao Wei ₁ , Huixin Zhang ₁ , Zonghua Wang ₁

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Luminol 是一种经典的电化学发光 (ECL) 发光体。鲁米诺-O ₂ ECL系统存在一个问题，即溶解的O ₂转化为活性氧（ROS）的转化率低。在这项工作中，我们首先使用高强度聚焦超声（HIFU）预处理结合Ti ₃ C ₂ –TiO ₂构建了高灵敏度的鲁米诺-O ₂ ECL系统，用于特异性检测多核苷酸激酶（PNK）。一方面，HIFU 通过空化效应原位产生 ROS 作为共反应物，以促进鲁米诺发射。另一方面，Ti ₃ C ₂ –TiO _2通过Ti₃原位制备C ₂作为还原剂，可聚集催化HIFU原位产生的ROS。此外，Ti ₃ C ₂ -TiO ₂表面的Ti可通过螯合作用与磷酸基团结合，从而实现对PNK的高特异性检测。该传感器线性范围为1.0 × 10 ^–5至10.0 U mL ^–1，检出限为1.48 × 10 ^–7 U mL ^–1，优于现有的大多数方法。传感器在 HeLa 细胞裂解物中的性能测量结果令人满意。所设计的ECL生物传感器在生物分析和临床诊断方面具有潜在的应用价值。

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High-Intensity Focused Ultrasound Combined with Ti3C2–TiO2 to Enhance Electrochemiluminescence of Luminol for the Sensitive Detection of Polynucleotide Kinase

Luminol is a classic electrochemiluminescence (ECL) luminophore. The luminol–O₂ ECL system suffers from a problem, that is, the conversion rate of dissolved O₂ into reactive oxygen species (ROS) is low. In this work, we used high-intensity focused ultrasound (HIFU) pretreatment combined with Ti₃C₂–TiO₂ to construct a highly sensitive luminol–O₂ ECL system for the specific detection of polynucleotide kinase (PNK) first. On the one hand, HIFU generated ROS in situ as a coreactant via the cavitation effect to boost the luminol emission. On the other hand, Ti₃C₂–TiO₂ was prepared in situ via Ti₃C₂ as a reducing agent, and it can aggregate and catalyze ROS generated in situ by HIFU. Moreover, the Ti on the Ti₃C₂–TiO₂ surface could bind to phosphate groups through chelation, thereby realizing highly specific detection of PNK. The sensor has a linear relationship range of 1.0 × 10^–5 to 10.0 U mL^–1, and the limit of detection is 1.48 × 10^–7 U mL^–1, which is superior to most existing methods. The sensor performance in HeLa cell lysate was measured with a satisfactory result. The designed ECL biosensor has potential applications in biological analysis and clinical diagnosis.

更新日期：2023-01-11

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