植物来源的虾青素生物合成途径在解脂耶氏酵母中的异源表达用于糖基化虾青素的生产,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

植物来源的虾青素生物合成途径在解脂耶氏酵母中的异源表达用于糖基化虾青素的生产
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-01-11 , DOI: 10.1021/acs.jafc.2c08153
Jing Chen _{1,

2} , Ruiling Zhang ₃ , Guilin Zhang _{1,

2} , Zhen Liu _{1,

2} , Hong Jiang _{1,

2} , Xiangzhao Mao _{1,

2,

4}

Affiliation

虾青素是一种高价值的红色素和抗氧化剂，广泛应用于制药、化妆品和食品行业。然而，虾青素的疏水性导致其生物利用度低。糖基化可以显着增加虾青素的水溶性，从而提高其生物利用度、光稳定性和生物活性。在这项研究中，我们首次报道了解脂耶氏酵母中糖基化虾青素的异源生产。通过适当去除叶绿体转运肽，来自Adonis aestivalis的类胡萝卜素 4-羟基-β-环 4-脱氢酶 (HBFD) 和类胡萝卜素 β-环 4-脱氢酶 (CBFD) 在产 β-胡萝卜素的 Y. lipolytica中表达菌株，导致虾青素产量为 0.59 mg/L，0.05 mg/g DCW。这是首次在酵母中成功构建植物来源的虾青素合成途径。CBFD 和 HBFD 的模块化组装、更换 CBFD 上游的启动子、增加前体 β-胡萝卜素供应以及调节 CBFD 和 HBFD 的表达导致虾青素产量增加 4.9 倍 (3.46 mg/L)。最后，将Pantoea ananatis ATCC 19321的crtX引入到虾青素生产菌株中，实现了糖化虾青素的生产，产量达到1.47 mg/L，这是迄今为止报道的微生物生产糖化虾青素的最高产量。

"点击查看英文标题和摘要"

Heterologous Expression of the Plant-Derived Astaxanthin Biosynthesis Pathway in Yarrowia lipolytica for Glycosylated Astaxanthin Production

Astaxanthin is a high-value red pigment and antioxidant widely used in the pharmaceutical, cosmetic, and food industries. However, the hydrophobicity of astaxanthin causes its low bioavailability. Glycosylation can substantially increase the water solubility of astaxanthin, thus enhancing its bioavailability, photostability, and biological activities. In this study, we report for the first time the heterologous production of glycosylated astaxanthin in Yarrowia lipolytica. By appropriate removal of the chloroplast transit peptide, carotenoid 4-hydroxy-β-ring 4-dehydrogenase (HBFD) and carotenoid β-ring 4-dehydrogenase (CBFD) from Adonis aestivalis were expressed in a β-carotene-producing Y. lipolytica strain, resulting in astaxanthin production with a yield of 0.59 mg/L, 0.05 mg/g DCW. This is the first time to successfully construct a plant-derived astaxanthin synthesis pathway in yeast. Modularized assembly of CBFD and HBFD, replacement of the promoter upstream CBFD, increasing the precursor β-carotene supply, and regulating the expressions of CBFD and HBFD led to a 4.9-fold increase in astaxanthin production (3.46 mg/L). Finally, introduction of crtX from Pantoea ananatis ATCC 19321 into the astaxanthin-producing strain enabled glycosylated astaxanthin production, and the yield reached 1.47 mg/L, which is the highest yield of microbially produced glycosylated astaxanthin reported to date.

更新日期：2023-01-11

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南