用于 siRNA 递送的具有与激发无关的长波长发射的树枝状大分子修饰的碳纳米粒子,ACS Applied Nano Materials

当前位置： X-MOL 学术 › ACS Appl. Nano Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于 siRNA 递送的具有与激发无关的长波长发射的树枝状大分子修饰的碳纳米粒子
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-01-09 , DOI: 10.1021/acsanm.2c04598
Fangdi Wei ₁ , Yuan Yao ₂ , Qiutong Chen ₂ , Jiamei Song ₂ , Zhen Xie ₂ , Li Ai ₂ , Guanhong Xu ₁ , Yao Cen ₁ , Yuhui Lin ₁ , Jing Yang ₁ , Qin Hu ₁ , Rui Li ₁

Affiliation

受碳纳米粒子 (CNP) 和聚酰胺胺 (PAMAM) 树枝状大分子独特优点的启发，我们合成了 PAMAM 改性的 CNP (CNPs-PAMAM)。首先，使用柠檬酸和 1,2,4-三氨基苯制备 CNP。然后，PAMAM 被锚定在 CNP 的表面上。PAMAM的结构在后修饰的温和条件下保持不变，使PAMAM保持其优异的载药能力。特别是，CNPs-PAMAM 具有与激发无关的长波长发射的特殊光学特性。激发无关发射可以保证生物成像的灵敏度。长波长发射可以避免生物基质的自发荧光干扰，具有良好的组织穿透性。进一步的研究表明，CNPs-PAMAM 能够静电结合小干扰 RNA (siRNA) 并将其凝聚成稳定的纳米复合物。与 CNPs-PAMAM 复合的 siRNA 具有增强的抗降解性和增强的细胞摄取。此外，靶向组蛋白脱乙酰酶 2 (HDAC2) 的 CNPs-PAMAM 复合 siRNA 可以有效地递送到神经元样 PC-12 细胞中，并发挥其基因沉默作用。精确抑制所谓的不可药物靶标 HDAC2 为缺血性中风治疗开辟了一种新策略。因此，CNPs-PAMAM 提供了一种很有前途的递送载体来释放 siRNA 的治疗潜力。靶向组蛋白脱乙酰酶 2 (HDAC2) 的 CNPs-PAMAM 复合 siRNA 可以有效地递送到神经元样 PC-12 细胞中，并发挥其基因沉默作用。精确抑制所谓的不可药物靶标 HDAC2 为缺血性中风治疗开辟了一种新策略。因此，CNPs-PAMAM 提供了一种很有前途的递送载体来释放 siRNA 的治疗潜力。靶向组蛋白脱乙酰酶 2 (HDAC2) 的 CNPs-PAMAM 复合 siRNA 可以有效地递送到神经元样 PC-12 细胞中，并发挥其基因沉默作用。精确抑制所谓的不可药物靶标 HDAC2 为缺血性中风治疗开辟了一种新策略。因此，CNPs-PAMAM 提供了一种很有前途的递送载体来释放 siRNA 的治疗潜力。

"点击查看英文标题和摘要"

Dendrimer-Modified Carbon Nanoparticles with Excitation-Independent Long Wavelength Emission for siRNA Delivery

Inspired by the distinctive merits of carbon nanoparticles (CNPs) and poly(amidoamine) (PAMAM) dendrimers, we synthesized PAMAM modified CNPs (CNPs-PAMAM). First, CNPs were prepared using citrate acid and 1, 2, 4-triaminobenzene. Then, PAMAM was anchored on the surface of CNPs. The structure of PAMAM would remain unchanged under mild conditions of the postmodification method, making PAMAM retain its excellent drug carrying ability. Particularly, CNPs-PAMAM had exceptional optical properties of excitation-independent long wavelength emission. Excitation-independent emission can ensure the sensitivity of bioimaging. Long wavelength emission can avoid the autofluorescence interference of biological matrixes and have good tissue penetration. Further studies demonstrated that CNPs-PAMAM were able to electrostatically bind and condense small interfering RNA (siRNA) into stabilized nanocomplexes. The siRNA complexed with CNPs-PAMAM was endowed with an increased resistance against degradation and an enhanced cellular uptake. Moreover, CNPs-PAMAM-complexed siRNA targeting histone deacetylase 2 (HDAC2) could be effectively delivered into the neuron-like PC-12 cells, and exert its gene-silencing effect. Precisely inhibiting the so-called undruggable target HDAC2 opens a novel strategy for ischemic stroke treatment. Thus, CNPs-PAMAM offer a promising delivery carrier to unleash the siRNA’s therapeutic potential.

更新日期：2023-01-09

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南