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Rice Defense Responses Orchestrated by Oral Bacteria of the Rice Striped Stem Borer, Chilo suppressalis
Rice ( IF 4.8 ) Pub Date : 2023-01-09 , DOI: 10.1186/s12284-022-00617-w
Rongrong Xue , Qing Li , Ruiqing Guo , Hui Yan , Xueyang Ju , Lu Liao , Rensen Zeng , Yuanyuan Song , Jie Wang

Plant defenses in response to chewing insects are generally regulated by jasmonic acid (JA) signaling pathway, whereas salicylic acid (SA) signaling is mainly involved in plant defense against biotrophic pathogens and piercing-sucking insects. Previous studies showed that both JA- and SA-related defenses in rice plants were triggered by the infestation of the rice striped stem borer (SSB, Chilo suppressalis), a destructive pest causing severe damage to rice production. Herbivore-associated microbes play an important role in modulating plant–insect interaction, and thus we speculate that the SSB symbiotic microbes acting as a hidden player may cause this anomalous result. The antibiotics (AB) treatment significantly depressed the performance of field-collected SSB larvae on rice plants, and reduced the quantities of bacteria around the wounds of rice stems compared to non-AB treatment. In response to mechanical wounding and oral secretions (OS) collected from non-AB treated larvae, rice plants exhibited lower levels of JA-regulated defenses, but higher levels of SA-regulated defenses compared to the treatment of OS from AB-treated larvae determined by using a combination of biochemical and molecular methods. Among seven culturable bacteria isolated from the OS of SSB larvae, Enterobacter and Acinetobacter contributed to the suppression of JA signaling-related defenses in rice plants, and axenic larvae reinoculated with these two strains displayed better performance on rice plants. Our findings demonstrate that SSB larvae exploit oral secreted bacteria to interfere with plant anti-herbivore defense and avoid fully activating the JA-regulated antiherbivore defenses of rice plants.



中文翻译:


水稻二化螟口腔细菌协调的水稻防御反应



植物对咀嚼昆虫的防御通常由茉莉酸(JA)信号通路调节,而水杨酸(SA)信号通路主要参与植物对生物营养病原体和刺吸式昆虫的防御。先前的研究表明,水稻植株中与 JA 和 SA 相关的防御都是由水稻二化螟 (SSB, Chilo secondaryis ) 的侵扰触发的,这是一种破坏性害虫,对水稻生产造成严重损害。草食动物相关微生物在调节植物-昆虫相互作用中发挥着重要作用,因此我们推测SSB共生微生物作为隐藏的参与者可能导致了这种异常结果。与非AB处理相比,抗生素(AB)处理显着降低了田间收集的SSB幼虫在水稻植株上的表现,并减少了水稻茎伤口周围的细菌数量。与从AB处理的幼虫中收集的机械损伤和口腔分泌物(OS)相比,水稻植株表现出较低水平的JA调节的防御,但与来自AB处理的幼虫的OS处理相比,表现出较高水平的SA调节的防御通过结合使用生物化学和分子方法。从SSB幼虫的OS中分离出的七种可培养细菌中,肠杆菌不动杆菌有助于抑制水稻植株中与JA信号相关的防御,并且用这两种菌株重新接种的无菌幼虫在水稻植株上表现出更好的性能。我们的研究结果表明,SSB 幼虫利用口腔分泌的细菌来干扰植物的抗食草动物防御,并避免完全激活 JA 调节的水稻植物的抗食草动物防御。

更新日期:2023-01-10
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