Parishin compounds are rare polyphenolic glucosides mainly found in the rhizome of the traditional Chinese medicinal plant, Gastrodia elata. These constituents are reported to have several biological and pharmacological activities. In the present study, two novel parishin derivatives not previously reported as plant-based phytochemicals were identified from a twig of Maclura tricuspidata (MT) and two new compounds were elucidated as 1-(4-(β-d-glucopyranosyloxy)benzyl)-3-hydroxy-3-methylpentane-1,5-dioate (named macluraparishin E) and 1,3-bis(4-(β-d-glucopyranosyloxy)benzyl)-3-hydroxy-3-methylpentane- 1,5-dioate (macluraparishin C), based on the experimental data obtained by UV-Visible (UV-Vis) spectroscopy, high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) and nuclear magnetic resonance (NMR) spectroscopy. Additionally, gastrodin, parishin A and parishin B were positively identified by spectroscopic evidence and the comparison of HPLC retention time with the corresponding authentic standards. Gastrodin, parishin A and parishin B, macluraparishin E and macluraparishin C were found to be the most abundant constituents in the MT twig. The compositions and contents of these constituents were found to vary depending on the different parts of the MT plant. In particular, the contents of parishin A, parishin B, macluraparishin C and macluraparishin E were higher in the twig, bark and root than in the leaves, xylem and fruit.

中文翻译：

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三尖吻合小枝中新型 Parishin 化合物的鉴定及不同部位 Parishin 衍生物的比较分析。

Parishin 化合物是一种罕见的多酚糖苷，主要存在于传统中药植物天麻的根茎中。据报道，这些成分具有多种生物学和药理学活性。在本研究中，两种新的 parishin 衍生物以前未被报道为基于植物的植物化学物质，从三尖瓣 (MT) 的树枝中鉴定出来，两种新化合物被阐明为 1-(4-(β-d-glucopyranosyloxy)benzyl)- 3-hydroxy-3-methylpentane-1,5-dioate（命名为 macluraparishin E）和 1,3-bis(4-(β-d-glucopyranosyloxy)benzyl)-3-hydroxy-3-methylpentane- 1,5-dioate (macluraparishin C)，基于通过紫外-可见 (UV-Vis) 光谱获得的实验数据，高效液相色谱-四极杆飞行时间质谱 (HPLC-QTOF-MS) 和核磁共振 (NMR) 光谱。此外，天麻素、parishin A 和 parishin B 通过光谱证据和 HPLC 保留时间与相应真实标准品的比较得到肯定鉴定。发现天麻素、parishin A 和 parishin B、macluraparishin E 和 macluraparishin C 是 MT 树枝中最丰富的成分。发现这些成分的组成和含量因 MT 植物的不同部分而异。其中，parishin A、parishin B、macluraparishin C和macluraparishin E在嫩枝、树皮和根中的含量高于叶、木质部和果实。parishin A 和 parishin B 通过光谱证据和 HPLC 保留时间与相应真实标准的比较得到肯定鉴定。发现天麻素、parishin A 和 parishin B、macluraparishin E 和 macluraparishin C 是 MT 树枝中最丰富的成分。发现这些成分的组成和含量因 MT 植物的不同部分而异。其中，parishin A、parishin B、macluraparishin C和macluraparishin E在嫩枝、树皮和根中的含量高于叶、木质部和果实。parishin A 和 parishin B 通过光谱证据和 HPLC 保留时间与相应真实标准的比较得到肯定鉴定。发现天麻素、parishin A 和 parishin B、macluraparishin E 和 macluraparishin C 是 MT 树枝中最丰富的成分。发现这些成分的组成和含量因 MT 植物的不同部分而异。其中，parishin A、parishin B、macluraparishin C和macluraparishin E在嫩枝、树皮和根中的含量高于叶、木质部和果实。发现这些成分的组成和含量因 MT 植物的不同部分而异。其中，parishin A、parishin B、macluraparishin C和macluraparishin E在嫩枝、树皮和根中的含量高于叶、木质部和果实。发现这些成分的组成和含量因 MT 植物的不同部分而异。其中，parishin A、parishin B、macluraparishin C和macluraparishin E在嫩枝、树皮和根中的含量高于叶、木质部和果实。