长双歧杆菌 CCFM1206 促进萝卜硫素向萝卜硫素的生物转化，有助于改善小鼠的葡聚糖-硫酸钠-诱导的结肠炎,Journal of Agricultural and Food Chemistry

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长双歧杆菌 CCFM1206 促进萝卜硫素向萝卜硫素的生物转化，有助于改善小鼠的葡聚糖-硫酸钠-诱导的结肠炎
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-01-05 , DOI: 10.1021/acs.jafc.2c07090
Jiaying Wu _{1,

2} , Shumao Cui _{1,

2} , Xin Tang _{1,

2} , Qiuxiang Zhang _{1,

2} , Yan Jin ₃ , Jianxin Zhao _{1,

2} , Bingyong Mao _{1,

2} , Hao Zhang _{1,

2,

4}

Affiliation

富含西兰花种子提取物 (BSE) 的萝卜硫素通常是惰性的，但可以通过肠道细菌生物转化为活性萝卜硫素。本研究旨在筛选具有萝卜硫苷代谢能力的益生菌，探讨菌株与BSE联合对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的影响。从健康成人粪便中分离出长双歧杆菌CCFM1206。超高效液相色谱 Q Exactive 质谱分析表明，长双歧杆菌CCFM1206在体外发酵的 BSE 上清液中存在萝卜硫素、萝卜硫素-l-半胱氨酸和芥红素。BSE 和B. longum的联合和单独干预CCFM1206 用于探索对 DSS 诱导的结肠炎的影响。结果表明，长双歧杆菌CCFM1206 和 BSE 的组合可以改善结肠炎症状，减轻结肠炎症反应和氧化应激，并保护 DSS 诱导小鼠的肠道屏障。与单独 BSE 干预相比，B. longum CCFM1206 和 BSE 的联合干预促进了小鼠结肠中萝卜硫素和萝卜硫素-N-乙酰半胱氨酸的产生，从 220.88 ± 19.81 到 333.99 ± 36.46 nmol/g 和从 232.04 ± 26.48 到 297.50分别为 ± 40.08 nmol/g 干重粪便。根据定量逆转录聚合酶链反应和免疫组织化学分析，长双歧杆菌CCFM1206和BSE有效激活了Nrf2信号通路相关基因的转录和表达。这些结果旨在阐明益生菌可以提高体内膳食植物化学物质的生物活性，并且这种组合具有治疗结肠炎的潜力。

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Bifidobacterium longum CCFM1206 Promotes the Biotransformation of Glucoraphanin to Sulforaphane That Contributes to Amelioration of Dextran-Sulfate-Sodium-Induced Colitis in Mice

Glucoraphanin, rich in broccoli seed extract (BSE), is generally inert but can be biotransformed into active sulforaphane by gut bacteria. This study aimed to screen probiotics with glucoraphanin-metabolizing ability and explore the effect of a combination of strain and BSE on colitis induced by dextran sulfate sodium (DSS) in mice. Bifidobacterium longum CCFM1206 was isolated from healthy adult feces. Ultra-high-performance liquid chromatography Q Exactive mass spectrometry analysis revealed the presence of sulforaphane, sulforaphane–l-cysteine, and erucin in the BSE supernatant fermented by B. longum CCFM1206 in vitro. Combined and individual interventions of BSE and B. longum CCFM1206 were applied to explore the effects on DSS-induced colitis. The results suggested that the combination of B. longum CCFM1206 and BSE could ameliorate colitis symptoms, relieve colonic inflammatory reactions and oxidative stress, and protect the intestinal barrier in DSS-induced mice. In comparison to the BSE intervention alone, the combined intervention of B. longum CCFM1206 and BSE promoted the generation of sulforaphane and sulforaphane–N-acetylcysteine in mice colon from 220.88 ± 19.81 to 333.99 ± 36.46 nmol/g and from 232.04 ± 26.48 to 297.50 ± 40.08 nmol/g dry weight feces, respectively. According to quantitative reverse transcription polymerase chain reaction and immunohistochemical analysis, B. longum CCFM1206 and BSE effectively activated the transcription and expression of genes related to the Nrf2 signaling pathway. These results were intended to elucidate that probiotics could elevate the bioactivity of dietary phytochemicals in vivo, and the combination had potential for therapeutic treatment of colitis.

更新日期：2023-01-05

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