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Enhanced fluorimetric detection of diphenylpyraline HCl using micelle and cyclodextrin mediated approach: Spectrofluorimetric and micellar liquid chromatographic application for either single or combined formulation with caffeine and paracetamol
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2023-01-05 , DOI: 10.1016/j.saa.2023.122317
Sherin F Hammad 1 , Samah F El-Malla 1 , Basma Z El-Khateeb 1
Affiliation  

Highly sensitive micellar spectrofluorimetric method (Method I) has been developed and validated for the determination of diphenylpyraline HCl in pharmaceutical tablets and in plasma. Sodium dodecyl sulfate improves the intensity of fluorescence of diphenylpyraline at 286 nm at pH 5 that allow its determination in plasma at nano-level. the mean percent recovery ± S.D was 99.719 ± 0.338 in plasma. In addition, Green cyclodextrin-modified micellar liquid chromatographic method (Method II) has been developed and validated for simultaneous determination of diphenylpyraline, paracetamol and caffeine using cyclodextrin micellar mobile phase consisted of 30 mM Brij*35, 0.5 mM hydroxypropyl β-cyclodextrin and phosphate buffer pH 4: MeOH (95:5, %v/v) that allows their simultaneous determination with enhanced spectrofluorimetric detection of diphenylpyraline. Method II was effectively applied for the simultaneous determination of diphenylpyraline, paracetamol and caffeine in a ternary laboratory prepared mixture which contained all possible excipients with mean percent recoveries ± S.D of 100.176 ± 1.008, 101.166 ± 0.415 and 100.708 ± 1.836, respectively. Linearity range for Method I was 0.1–1 μg. mL−1 for diphenylpyraline and for Method II was 0.3–50, 25–350, and 0.5–50 for caffeine, paracetamol and diphenylpyraline, respectively. Method I was also applied in spiked human plasma with linearity range 0.2–0.5 μg. mL−1. The methods are verified to have excellent greenness.



中文翻译:

使用胶束和环糊精介导的方法增强二苯基吡拉林盐酸盐的荧光检测:分光荧光法和胶束液相色谱法在咖啡因和扑热息痛的单一或组合制剂中的应用

已经开发并验证了高灵敏度的胶束荧光分光光度法(方法 I),用于测定药片和血浆中的二苯基吡拉林盐酸盐。Sodium dodecyl sulfate 提高了二苯基吡拉林在 pH 5 时在 286 nm 处的荧光强度,使其能够在血浆中进行纳米水平的测定。血浆中的平均回收率 ± SD 为 99.719 ± 0.338。此外,开发并验证了绿色环糊精改性胶束液相色谱法(方法 II),使用由 30 mM Brij*35、0.5 mM 羟丙基 β-环糊精和磷酸盐组成的环糊精胶束流动相同时测定二苯基吡拉林、扑热息痛和咖啡因pH 4 缓冲液:MeOH (95:5, %v/v),这使得它们可以通过增强的二苯基吡喃分光光度法检测同时测定。方法 II 有效地用于同时测定三元实验室制备的混合物中二苯基吡拉林、扑热息痛和咖啡因的含量,该混合物包含所有可能的赋形剂,平均百分比回收率 ± SD 分别为 100.176 ± 1.008、101.166 ± 0.415 和 100.708 ± 1.836。方法 I 的线性范围为 0.1–1 μg。毫升对于咖啡因、扑热息痛和二苯基吡拉林,二苯基吡拉林和方法 II 的-1分别为 0.3–50、25–350 和 0.5–50。方法 I 也适用于线性范围为 0.2–0.5 μg 的加标人血浆。毫升-1。这些方法被证实具有极好的绿色性。

更新日期:2023-01-07
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