Development of an in Vitro Digestion System to Study the Bioavailability and Bioreactivity of Zinc Sulfate and Zn-Bioplex in Fish Using the RTgutGC Cell Line,ACS Food Science & Technology

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Development of an in Vitro Digestion System to Study the Bioavailability and Bioreactivity of Zinc Sulfate and Zn-Bioplex in Fish Using the RTgutGC Cell Line
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2023-01-05 , DOI: 10.1021/acsfoodscitech.2c00307
Dean Oldham ₁ , William Dudefoi _{1,

2} , Matteo Minghetti ₁

Affiliation

Here, we report the development of a novel in vitro digestion system that mimics the digestive process in fish and uses the RTgutGC cell line, a model of the rainbow trout (Oncorhynchus mykiss) intestine, to study the bioavailability, bioreactivity, and toxicity of Zn added as an inorganic (ZnSO₄) and organic zinc compound (Zn-Bioplex). Our results showed that, before digestion, Zn-Bioplex was more bioavailable than ZnSO₄. However, after in vitro digestion, ZnSO₄ and Zn-Bioplex accumulated and were transported equally across the RTgutGC cell epithelium. However, Zn-Bioplex was shown to be less bioreactive than ZnSO₄, suggesting that peptide complexation might reduce the intracellular release of zinc as shown by a reduced induction of metallothionein and ZnT1 mRNA levels. This novel method shows promise as a standardized in vitro fish digestion model to study the interactions occurring at the lumen of the intestine and the bioavailability and toxicity of digested compounds in the fish gut.

中文翻译：

开发体外消化系统，使用 RTgutGC 细胞系研究鱼类中硫酸锌和 Zn-Bioplex 的生物利用度和生物反应性

在这里，我们报告了一种新型体外消化系统的开发，该系统模拟鱼类的消化过程，并使用 RTgutGC 细胞系（一种虹鳟鱼 ( Oncorhynchus mykiss ) 肠道模型）来研究锌的生物利用度、生物反应性和毒性作为无机 (ZnSO ₄ ) 和有机锌化合物 (Zn-Bioplex) 添加。我们的结果表明，在消化之前，Zn-Bioplex 比 ZnSO ₄具有更高的生物利用度。然而，在体外消化后，ZnSO ₄和 Zn-Bioplex 积累并在 RTgutGC 细胞上皮细胞中平均转运。然而，Zn-Bioplex 的生物反应性低于 ZnSO ₄，这表明肽络合可能会减少锌的细胞内释放，如金属硫蛋白和 ZnT1 mRNA 水平的诱导减少所示。这种新方法有望作为一种标准化的体外鱼类消化模型，用于研究肠腔发生的相互作用以及鱼肠中消化化合物的生物利用度和毒性。

更新日期：2023-01-05

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