The luminescent carbon dots with peroxidase mimicking property had attracted considerable attention in biomedical field. In this work, iron-doped carbon dots (Fe-CDs) were prepared by one-pot hydrothermal method with 5, 10, 15, 20-tetra (4-borate phenyl)−21H, 23H-porphyrin Fe (II) (Fe-TBPP) as precursor. The obtained Fe-CDs emitted intense blue luminescence under ultraviolet light irradiation. Moreover, the Fe-CDs exhibited remarkable peroxidase mimicking property, which can efficiently catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into blue ox-TMB in the presence of hydrogen peroxide (H₂O₂). More importantly, the emission of Fe-CDs could be gradually quenched with the addition of H₂O₂. Based on these phenomena, a new optical dual-mode (colorimetric and fluorometric) method for the detection of H₂O₂ and glucose was successfully established. The detection limits of glucose were calculated to be 3.86 and 7.27 μM (S/N = 3) respectively based on the colorimetric and fluorometric methods. Furthermore, we combined this dual-mode detection method with smartphone imaging. The colorimetric and fluorescent images were collected by recognition software of smartphone, which were then transformed into the corresponding HSL values for quantitative determination of glucose. Finally, the dual-mode approach based on Fe-CDs was used for the detection of glucose content in human serum, demonstrating the potential application of carbon dots in the biological area.

具有模拟过氧化物酶特性的发光碳点在生物医学领域引起了广泛关注。在这项工作中，通过一锅法水热法制备了铁掺杂碳点（Fe-CDs），其中 5, 10, 15, 20-四（4-硼酸苯基）-21H, 23H-卟啉 Fe (II) (Fe -TBPP) 作为前体。获得的 Fe-CDs 在紫外光照射下发出强烈的蓝色荧光。此外，Fe-CDs表现出显着的过氧化物酶模拟特性，可在过氧化氢（H ₂ O ₂）存在下有效催化3, 3', 5, 5'-四甲基联苯胺（TMB）氧化成蓝色ox-TMB . 更重要的是，Fe-CDs 的发射可以通过添加 H ₂ O _{2逐渐淬灭}. 基于这些现象，成功建立了检测H ₂ O _{2和葡萄糖的新型光学双模式（比色法和荧光法）方法。}根据比色法和荧光法计算出葡萄糖的检测限分别为 3.86 和 7.27 μM (S/N = 3)。此外，我们将这种双模式检测方法与智能手机成像相结合。通过智能手机的识别软件采集比色和荧光图像，然后将其转化为相应的HSL值，用于葡萄糖的定量测定。最后，基于Fe-CDs的双模式方法被用于检测人血清中的葡萄糖含量，展示了碳点在生物领域的潜在应用。