<br>绿色 AOT 反胶束作为碱性磷酸酶的纳米反应器。氢键在水和酶、反应产物以及反胶束界面之间“跳舞”,RSC Advances

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绿色 AOT 反胶束作为碱性磷酸酶的纳米反应器。氢键在水和酶、反应产物以及反胶束界面之间“跳舞”
RSC Advances ( IF 3.9 ) Pub Date : 2023-01-04 , DOI: 10.1039/d2ra06296h
Gustavo A Monti _{1,

2} , R Darío Falcone ₁ , Fernando Moyano ₁ , N Mariano Correa ₁

Affiliation

在这项工作中，我们研究了 1,4-双-2-乙基己基磺基琥珀酸酯/月桂酸甲酯和 1,4-双-2-乙基己基磺基琥珀酸酯/肉豆蔻酸异丙酯反胶束中封装的水对 1-萘基酶水解的影响通过碱性磷酸酶磷酸化。我们的结果表明，该酶在所研究的生物相容性反胶束中具有活性，并且米氏动力学模型在所有系统中都有效。我们发现所研究的两种胶束系统对 pH 值都有特殊的行为，外部溶剂渗透到界面对于理解这种影响至关重要。月桂酸甲酯不会破坏界面，也不需要控制 pH 值，因为胶束中心的碱性磷酸酶总是类似地溶剂化。相反，肉豆蔻酸异丙酯破坏界面，使得水和1-萘酚分子不能与表面活性剂的极性头形成氢键相互作用。然后，当水的 pH = 7 时，1-萘酚移动到抑制碱性磷酸酶的界面，当水的 pH = 10 时，这是观察不到的。我们的研究表明，pH 的概念不能直接用于密闭环境。此外，我们的研究在酶催化的反胶束中发生的反应领域具有重要意义。

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Green AOT reverse micelles as nanoreactors for alkaline phosphatase. The hydrogen bond “dances” between water and the enzyme, the reaction product, and the reverse micelles interface

In this work, we present an investigation of the influence of water encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/methyl laurate and 1,4-bis-2-ethylhexylsulfosuccinate/isopropyl myristate reverse micelles on the enzymatic hydrolysis of 1-naphthyl phosphate by alkaline phosphatase. Our results show that the enzyme is active in the biocompatible reverse micelles studied and that the Michaelis–Menten kinetic model is valid in all systems. We found that both micellar systems studied have a particular behavior toward pH and that the penetration of external solvents into the interfaces is crucial to understanding the effect. Methyl laurate does not disrupt the interface and is not necessary to control the pH value since alkaline phosphatase in the center of the micelles is always solvated similarly. In contrast, isopropyl myristate disrupts the interfaces so that the water and 1-naphthol molecules cannot form hydrogen bond interactions with the polar head of the surfactant. Then, when the water is at pH = 7, the 1-naphthol moves away to the interfaces inhibiting alkaline phosphatase which is not observable when the water is at pH = 10. Our study shows that the concept of pH cannot be used directly in a confined environment. In addition, our research is of great importance in the field of reactions that occur in reverse micelles, catalyzed by enzymes.

更新日期：2023-01-05

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