The present work examined the effect exerted by tectoridin preventing oxygen glucose deprivation/reoxygenation (OGD/R) damage within PC12 cell. We incubated PC12 cells with Na₂S₂O₄ (10 mM) for 2 h, and tectoridin at different concentrations was then added; based on methyl-thiazolyl-tetrazolium (MTT) and lactate dehydrogenase (LDH) tests, the protection impact was tested. 2',7'-dicholorofluorescein diacetate (DCFH-DA), Fluo-3AM, and 5, 5', 6, 6' -tetrachloro-1, 1', 3, 3' -tetraethyl-imidacarbocyanine iodide (JC-1) staining, and Western blotting were used for determining reactive oxygen species (ROS) level, intracellular Ca²⁺ content, mitochondrial membrane potential (MMP) and the related proteins contents. As a result, tectoridin could improve the cell viability and inhibit the release of LDH. In-depth studies demonstrated that tectoridin limited the overproduction of ROS and intracellular Ca²⁺ content and increased MMP, which showed a close association with ROS-mediated mitochondrial function. Moreover, tectoridin hindered apoptosis based on the up-regulation of the expressions of p-AKT, Bcl-2/Bax and p-mTOR. Furthermore, the level of Nrf2 was also improved by treatment of tectoridin. In addition, the expression of Bcl-2/Bax, p-Akt, p-mTOR, Nrf2, HO-1, NQO1 and GCLM were reduced by LY294002 and the protective role of tectoridin was limited by LY294002. The results unambiguously suggested that tectoridin reduced OGD/R-caused damage to PC12 cells and might ensure neuroprotection by stimulating the PI3K/AKT signaling channel.

目前的工作检查了鸢尾花苷在 PC12 细胞内预防氧葡萄糖剥夺/复氧 (OGD/R) 损伤所发挥的作用。_{我们用Na 2} S ₂ O ₄ (10 mM)孵育PC12细胞2小时，然后加入不同浓度的鸢尾黄酮；基于甲基噻唑基四唑（MTT）和乳酸脱氢酶（LDH）测试，测试保护效果。2',7'-二氯荧光素二乙酸酯 (DCFH-DA)、Fluo-3AM 和 5, 5', 6, 6' -四氯-1, 1', 3, 3' - 四乙基-亚咪卡博花青碘化物 (JC-1)染色和 Western blotting 用于测定活性氧 (ROS) 水平、细胞内 Ca ²⁺含量、线粒体膜电位（MMP）及相关蛋白含量。因此，鸢尾花苷可以提高细胞活力并抑制 LDH 的释放。深入研究表明，鸢尾花苷限制了 ROS 和细胞内 Ca ^{2+的过量产生}含量和增加的 MMP，这表明与 ROS 介导的线粒体功能密切相关。此外，紫杉醇基于上调 p-AKT、Bcl-2/Bax 和 p-mTOR 的表达而阻碍细胞凋亡。此外，nrf2 的水平也通过 tectoridin 处理得到改善。此外，Bcl-2/Bax、p-Akt、p-mTOR、Nrf2、HO-1、NQO1 和 GCLM 的表达被 LY294002 降低，而保护作用被 LY294002 限制。结果明确表明，鸢尾花苷减少了 OGD/R 对 PC12 细胞造成的损伤，并可能通过刺激 PI3K/AKT 信号通道确保神经保护作用。