Scientific Reports ( IF 3.8 ) Pub Date : 2023-01-03 , DOI: 10.1038/s41598-022-27341-1
Lucía Blasco 1, 2 , Manuel González de Aledo 1, 2 , Concha Ortiz-Cartagena 1, 2 , Inés Blériot 1, 2 , Olga Pacios 1, 2 , María López 1, 2 , Laura Fernández-García 1, 2 , Antonio Barrio-Pujante 1, 2 , Marta Hernández-Garcia 2, 3 , Rafael Cantón 2, 3 , María Tomás 1, 2
|
Phage tail-like bacteriocins (PTLBs) are large proteomic structures similar to the tail phages. These structures function in bacterial competition by making pores in the membrane of their competitors. The PTLBs identified in Pseudomonas aeruginosa are known as R-type and F-type pyocins, which have a narrow spectrum of action. Their specificity is determined by the tail fiber and is closely related to the lipopolysaccharide type of the target competitor strain. In this study, the genome sequences of 32 clinical of P. aeruginosa clinical isolates were analysed to investigate the presence of R-type and F-type pyocins, and one was detected in all strains tested. The pyocins were classified into 4 groups on the basis of the tail fiber and also the homology, phylogeny and structure of the cluster components. A relationship was established between these groups and the sequence type and serotype of the strain of origin and finally the killing spectrum of the representative pyocins was determined showing a variable range of activity between 0 and 37.5%. The findings showed that these pyocins could potentially be used for typing of P. aeruginosa clinical isolates, on the basis of their genomic sequence and cluster structure, and also as antimicrobial agents.
中文翻译:

研究铜绿假单胞菌临床收集的 32 种新型噬菌体尾状细菌素(脓菌素)及其作为分型标记和抗菌剂的潜在用途
噬菌体尾样细菌素 (PTLB) 是类似于尾部噬菌体的大型蛋白质组结构。这些结构通过在竞争者的膜上制造孔隙在细菌竞争中发挥作用。在铜绿假单胞菌中鉴定出的 PTLB被称为 R 型和 F 型脓菌素,它们的作用范围很窄。它们的特异性由尾纤维决定,与目标竞争菌株的脂多糖类型密切相关。本研究对 32 个临床铜绿假单胞菌的基因组序列分析临床分离株以研究 R 型和 F 型脓菌素的存在,并在所有测试菌株中检测到一种。根据尾纤维以及簇组分的同源性、系统发育和结构,将绿脓素分为 4 个组。在这些组与起源菌株的序列类型和血清型之间建立了关系,最后确定了代表性脓菌素的杀灭谱,显示出 0 到 37.5% 之间的可变活性范围。研究结果表明,这些绿脓素有可能根据其基因组序列和簇结构用于铜绿假单胞菌临床分离株的分型,也可用作抗菌剂。