壳聚糖/海藻酸盐纳米颗粒缓释七叶皂苷 A 用于烧伤创面愈合,ACS Applied Nano Materials

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壳聚糖/海藻酸盐纳米颗粒缓释七叶皂苷 A 用于烧伤创面愈合
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-01-03 , DOI: 10.1021/acsanm.2c04714
Zhikang Zhu _{1,

2,

3} , Fang He _{1,

2,

3} , Huawei Shao _{1,

2,

3} , Jiaming Shao _{1,

2,

3} , Qiong Li _{1,

2,

3} , Xingang Wang _{1,

2,

3} , Haitao Ren _{1,

2,

3} , Chuangang You _{1,

2,

3} , Zhongtao Zhang _{1,

2,

3,

4} , Chunmao Han _{1,

2,

3}

Affiliation

烧伤仍然是全球公共卫生面临的最具破坏性的负担之一。由烧伤和真皮支架移植引起的炎症通常会导致烧伤创面愈合延迟。Esculentoside A (EsA) 具有很强的抗炎能力，是一种可能有助于治疗烧伤的可用药物。然而，EsA较差的稳定性和毒性限制了其临床应用。在本研究中，我们构建了壳聚糖/藻酸盐纳米颗粒 (EsA-CS/ALG-NPs) 以提高可持续性并降低毒性，然后将制备的 EsA-CS/ALG-NPs 浸渍到胶原蛋白/壳聚糖支架 (EsA-CS/ ALG-NPs@CCS）。使用 Nanometrics 仪器评估修复纳米粒子的粒径、结构形态、热性能和化学相互作用，分别是差示扫描量热法、透射电子显微镜和傅立叶变换红外光谱。混合 EsA-CS/ALG-NPs@CCS 的物理特性、体外药物释放、生物相容性和 RAW 264.7 细胞的抗炎能力以及 SD 大鼠的体内烧伤伤口愈合研究得到了评估。结果表明，我们成功构建了CS/ALG-NPs，并优化了制备工艺，实现了最高的包封效率。EsA-CS/ALG-NPs@CCS杂化物具有降低的细胞毒性和EsA的持续释放，可以在体外减轻炎症，降低M1巨噬细胞的比例，增加M2巨噬细胞的比例。结果表明，5 μg EsA CS/ALG-NPs@CCS不仅可以减少炎性细胞因子的分泌，抑制M1巨噬细胞，还可以促进抗炎细胞因子的释放，激活M2巨噬细胞，从而实现烧伤创面的加速高质量愈合。最终。总之，我们的工作表明，EsA、纳米颗粒和支架的协同组合为治疗烧伤提供了一种有前途的策略。

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Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

Burn injury remains one of the most devastating burdens on global public health. The inflammation, caused by burn injury and transplantation of dermal scaffolds, often leads to delayed burn wound healing. Esculentoside A (EsA), with a strong anti-inflammatory capacity, is an available agent that might contribute to the treatment of burn wounds. However, the poor stability and toxicity of EsA limit its clinical application. In the present study, we constructed chitosan/alginate nanoparticles (EsA-CS/ALG-NPs) to improve sustainability and reduce toxicity followed by impregnation of the prepared EsA-CS/ALG-NPs into a collagen/chitosan scaffold (EsA-CS/ALG-NPs@CCS). The particle size, structural morphology, thermal properties, and chemical interaction of repaired nanoparticles were evaluated using the Nanometrics instrument, differential scanning calorimetry, transmission electron microscopy, and Fourier transform infrared spectroscopy, respectively. The hybrid EsA-CS/ALG-NPs@CCS was evaluated for physical characteristics, in vitro drug release, biocompatibility, and anti-inflammation capacity with RAW 264.7 cells and in vivo burn wound healing studies with SD rats. The results showed that we successfully constructed CS/ALG-NPs and optimized the preparation process to achieve the highest encapsulation efficiency. The hybrid EsA-CS/ALG-NPs@CCS, with reduced cytotoxicity and sustained release of EsA, could alleviate inflammation, decrease the ratio of M1 macrophages, and increase the proportion of M2 macrophages in vitro. It was demonstrated that 5 μg EsA CS/ALG-NPs@CCS not only reduces inflammatory cytokines secretion and inhibits M1 macrophages but also promotes the release of anti-inflammatory cytokines and activates M2 macrophages, thereby achieving accelerated and high-quality healing of burn wounds ultimately. In summary, our work suggests that the synergistic combination of EsA, nanoparticles, and scaffolds provided a promising strategy for treating burn injuries.

更新日期：2023-01-03

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